硫氧还蛋白及其还原酶在新生鼠高氧肺损伤中的表达  

Expression of thioredoxin and thioredoxin reductase in hyperoxia-induced lung injury of newborn rats

作　　者：陈燕[1] 常立文[1] 李文斌[1] 蔡成[1] 刘伟[1] 单瑞艳[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院儿科,武汉430030

出　　处：《中国小儿急救医学》2008年第4期345-347,共3页Chinese Pediatric Emergency Medicine

基　　金：国家自然科学基金资助项目（30770944）;国家自然科学基金资助项目（30672261）

摘　　要：目的研究硫氧还蛋白（Trx）及其还原酶（TR）在早产新生鼠高氧肺损伤的肺组织中的表达变化及意义。方法早产新生SD大鼠，生后第一天随机分为空气组和高氧组，每组64只。两组分别于高氧或空气暴露后第4、7、10、14天提取肺组织总RNA，采取半定量逆转录聚合酶链反应（RT-PCR）测定Trx和TR mRNA表达；同时采用HE染色观察肺组织病理学变化，并进行辐射状肺泡计数（RAC）。结果病理学观察显示：与对照组比较，高氧组肺组织出现明显肺泡炎性改变和肺发育滞后，同时RAC值亦较空气组显著减少（P〈0．05）。高氧暴露各组Trx、TR mRNA表达较空气组均明显增强（P〈0．05），且二者表达强度分别于第10天和第7天达高峰。结论高氧上调肺组织Trx、TR表达；肺组织Trx系统表达增高可能在早产鼠高氧肺损伤的发病过程中发挥重要保护作用。Objective To investigate the changes and potential roles of the expression of thioredoxin and thioredoxin reductase in the premature newborn rat lung exposed to hyperoxia. Methods On the first day after delivery, the preterm SD rats were randomly divided into air group and hyperoxia group （both n = 64）. The rats were killed on day 4, 7, 10 and 14 respectively. Total RNA of lung was isolated and Trx and TR mRNA expression levels were detected by reverse transcription polymerase chain reaction（ RT-PCR）. The sections of lung were stained with HE method in order to assess lung histologic changes and examine lung radical alveolar counts （RAC）. Results Rats in hyperoxia group showed typical lung injury, which was characterized by alveolitis and delay of lung development. Compared with air group, RACs were significantly decreased in hyperoxia group （ P 〈 0.05 ）. RT-PCR results showed Trx and TR mRNA expression levels in hyperoxia group markedly increased, peaking at day 10 （ 1. 444 ± 0.142 vs 0. 609 ± 0. 078, P 〈 0.01 ） and day 7 （2. 035 ± 0. 160 vs 1. 325 ± 0.164, P 〈 0.01 ） respectively. Conclusion Trx and TR expression could be induced by hyperoxia, which might play an importantly protective role in the development of hyperoxia-induced lung injury.

关 键 词：高氧 早产 硫氧还蛋白 肺损伤 

分 类 号：R722.1[医药卫生—儿科]