辛芷鼻敏胶囊对变应性鼻炎大鼠血清NO和鼻粘膜iNOS表达的影响  

Effects of Xinzhi Bimin capsule on the content of nitric oxide in blood serum and expression of iNOS mRNA in nasal mucosa of rat model with allergic rhinitis

作　　者：姜孝芳[1] 高丽[1] 李惠武[1] 包晓玲[2] 刘江[1] 赵孟[1] 杨清[1] 

机构地区：[1]新疆医科大学基础医学院,乌鲁木齐830054 [2]甘肃省肿瘤医院,兰州730050

出　　处：《中国免疫学杂志》2008年第8期712-715,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金项目(No.30560144)

摘　　要：目的:探讨辛芷鼻敏胶囊对变应性鼻炎(Allergic rhinitis,AR)大鼠血清一氧化氮(NO)及鼻粘膜诱导型一氧化氮合酶(iNOS)mRNA表达的影响。方法:40只大鼠随机分为空白对照组、模型组、阳性对照组、实验组,用卵清蛋白建立变应性鼻炎大鼠模型,通过对造模的大鼠灌服辛芷鼻敏胶囊及与同类药物对照,用酶法检测血清一氧化氮水平及逆转录-多聚酶链反应(RT-PCR)半定量测定鼻粘膜诱导型一氧化氮合酶mRNA的表达。结果:实验药物组的血清NO的水平和iNOS mRNA的表达明显低于模型组及阳性对照组(P<0.05)。结论:辛芷鼻敏胶囊可能通过降低鼻粘膜iNOS mRNA的表达及血清NO含量,抑制大鼠AR发病过程NO的合成,进而减少嗜酸粒细胞(Eosinophil,EOS)的浸润而改善症状。Objective： To study the effect of Xinzhi Bimin capsule on the content of nitric oxide in serum and the expression of iNOS mRNA in nasal mucosa of allergic rhimtis, and to analyze the mechanism by pharmacodynamic action. Methods： Forty rats were randomly divided into four groups as the normal control group,model group,positive control group and experimental group. Ovalbumin sensitized rats used as the animals of AR took orally with the Xinzhi Bimin capsule. Nitric oxide in sera was measured by nitrate reductase. The expression of iNOS mRNA in nasal mucosa of the rats was observed by revers transcriptive polymerase chain reaction （RT-PCR） .Results： The content of nitric oxide in the sera of model group （57.28 ± 3.76） was higer than that in the experimental group （29.11 ± 2.63 ）, positive control group （ 45.80 ±6.47）and normal control group （17.40±3.76）（ P 〈 0.05）. The expression of iNOS mRNA in nasal mucosa of rats of model group （ 1.189 9 ±0.078 ） was higer than in the experimental group（0.764 8 ± 0.061）, positive control group （ 0. 973 2± 0. 055） （ P 〈 0.05）, and no expression in normal control group.The content of nitric oxide was lower in sera of the experimental group than in the model group（ P 〈0.05） and the positive control group.Moreover,the expression of iNOS mRNA in nasal mucosa of rats in the experimental group was lower compared to those in the model group and the positive control group. Conclusion：The result suggests that the capsule of Xinzhi Bimin can reduce the expression of iNOS mRNA in nasal mucosa of rats and down-regulate content of nitric oxide in sera, whereby to treat AR in rats.

关 键 词：辛芷鼻敏胶囊 NO INOS RT-PCR 

分 类 号：R976[医药卫生—药品] R765.22[医药卫生—药学]