甲壳低聚糖对THP-1源性巨噬细胞脂质转运相关基因表达的影响  

Effect of chitooligosaccharides on expression of lipid-traffic related genes in THP-1-derived macrophages

作　　者：曹运长[1,2] 黄春林[1] 文红波[1] 刘飞[2] 李邦良[1] 

机构地区：[1]南华大学生化与分子生物学教研室,湖南衡阳421001 [2]湖南工业大学绿色包装与生物纳米技术应用湖南省重点实验室,湖南株洲412008

出　　处：《中国生化药物杂志》2008年第4期225-228,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：湖南省自然科学基金(06JJ30016);绿色包装与生物纳米技术应用湖南省重点实验室开放基金(ZN06003)

摘　　要：目的观察甲壳低聚糖(COS)对离体培养的THP-1源性巨噬细胞脂质转运相关基因表达的影响,以初步探讨COS降低血脂的可能分子机制。方法离体培养人THP-1单核细胞,诱导分化成巨噬细胞后,用100μg/mL的COS处理THP-1源性巨噬细胞6,12,24,48 h,运用半定量RT-PCR方法检测其LDL受体、SR-BⅠ、ABCA1和CD36mRNA表达水平的变化。结果COS处理THP-1源性巨噬细胞24和48 h后,LDL受体mRNA的表达与对照组比较分别下调了15.6%(P<0.05)和30.7%(P<0.01);处理6,12,24,48 h后,SR-BⅠmRNA的表达与对照组比较分别上调了14.7%,23.0%,14.6%(P<0.05)和33.9%(P<0.01);处理6和12 h后,ABCA1 mRNA的表达与对照组比较分别上调了12.6%和14.5%(P<0.05);处理6和12 h后,CD36 mRNA的表达与对照组比较分别下调了25.5%和10.2%(P<0.05)。结论通过细胞离体培养实验表明,COS具有的降低血脂作用可能与其能上调巨噬细胞中AB-CA1和SR-BⅠmRNA表达水平以及降低LDL受体和CD36 mRNA水平有关。Purpose To investigate the effect of chitooligosaccharides （COS） on expression of lipid-traffic related genes in THP-1-derived macrophages in vitro, so as to clarify the mechanism that COS could decrease blood lipid level. Methods THP-1-derived macrophages were cultured and treated with COS （100μg/mL） for 6,12, 24 and 48 h respectively. Then LDL receptor, SR-BⅠ, ABCA1, and CD36 mRNA expression were determined by semi-quantity RT-PCR.Results Compared with control, COS significantly increased ABCA1 after 6 h and 12 h and SR-BⅠ mRNA expression after 6, 12, 24 and 48 h, and significantly decreased LDL receptor mRNA level after 24 h or 48 h and CD36 mRNA level after 6 h and 12 h. Conclusion COS could up-regulated ABCA1 and SR-BⅠ mRNA level, and down-regulated LDL receptor and CD36 mRNA expression in macrophage cells in vitro, which could be related with the activity of COS decreasing blood lipid level.

关 键 词：甲壳低聚糖 THP-1细胞 脂质转运 基因表达 

分 类 号：Q78[生物学—分子生物学]