RGD-tTF水基磁流体的制备及其活性研究  

Studies of preparation and properties of RGD-tTF water-based ferrofluids

作　　者：颜江华[1] 黄志平[2] 王生育[1] 王勇军[2] 李文珠[2] 

机构地区：[1]厦门大学医学院抗癌研究中心,福建厦门361005 [2]厦门大学生命科学学院,福建厦门361005

出　　处：《中国生化药物杂志》2008年第4期229-232,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：教育部和厦门大学出国留学人员启动基金资助

摘　　要：目的探讨RGD-tTF水基磁流体通过磁场和RGD多肽在体外对内皮细胞双靶向的功能。方法通过化学沉淀法以柠檬酸钠为表面活性剂制备水基磁流体(MnFe2O4),弱酸改性后与重组的RGD-tTF融合蛋白结合,利用H-600透射电镜观测纳米粒径,以SUQID鉴定磁性,用MTT法、因子X活化检测和流式细胞仪检测RGD-tTF磁流体生物活性。结果成功制备出的水基磁流体能在磷酸盐缓冲液中稳定分布且具有生物兼容性,实验表明RGD-tTF与水基磁流体结合后对RGD和tTF生物活性均无显著影响,并证实在磁场的作用下能实现了水基磁流体对RGD-tTF的定位作用。结论一种具有内皮细胞双靶向功能的RGD-tTF水基磁流体已制备成功。Purpose To study the property of RGD-tTF water-based ferrofluids double targeting EC304 ceils in vitro by magnet and RGD peptide. Methods Water-based ferrofluids （MnFe2O4） were prepared by chemical precipitation method using citrate as surfactant, dispersed in weak acid to create surface charges, and coated with recombinant RGD-tTF protein. Proteins coated ferrofluids were characterized by H-600 transmission electron microscopy（TEM）and Superconducting Quantum Interference Device（SQUID）, and its biological activities were analyzed by MTT assay, factor X activating test, and flow cytometry. Results The water-based ferrofluids were biocompatible, and can be stably distribute in PBS solution. After RGD-tTF protein binding ferrofluids, the biological activities of the RGD-tTF protein have not been changed significantly. The RGD-tTF ferrofluids can be delivered and localized Selectively in vitro by magnet. Conclusion RGD-tTF water-based ferrofluids with double targeting had been successfully prepared.

关 键 词：水基磁流体 生物兼容 靶向 内皮细胞 

分 类 号：R392.1[医药卫生—免疫学]