壳寡糖减弱乙醇对人胚胎肝细胞损伤的研究  

Protective effect of oligochitosan on ethanol induced injury in human embryonic hepatocytes

作　　者：许青松[1,2] 麻攀[1,2] 魏鹏[1,2] 窦江丽[1,2] 白雪芳[1] 杜昱光[1] 

机构地区：[1]中国科学院大连化学物理研究所天然产物与糖工程组,辽宁大连116023 [2]中国科学院研究生院,北京100049

出　　处：《中国生化药物杂志》2008年第4期259-261,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的研究壳寡糖减弱乙醇对人胚胎肝细胞(L02细胞)损伤的原因。方法采用MTT法检测壳寡糖减弱乙醇对L02细胞的损伤;结合RT-PCR和Western blot方法分析壳寡糖的保护作用。结果壳寡糖能明显降低乙醇对L02细胞的损伤(P<0.01),尤其是0.2 mg/mL的壳寡糖,而这种作用是通过降低p53基因和蛋白的表达来实现的。结论壳寡糖有保护L02细胞抵制乙醇损伤的作用。Purpose To study the protective effect of oligochitosan on injury induced by ethanol in human embryonic hepatocytes （L02 cells）. Methods MTT assay was used for evaluating protection of oligochitosan on L02 cells against ethanol ; RT-PCR and Western blot were used for explaining the reason of protection. Results Oligochitosan notably recovered the injury induced by ethanol （ P 〈 0.01 ）, especially the 0.2 mg/mL oligochitosan. The mechanism was possibly that oligochitosan down-regulated p53 expression. Conclusion Oligochitosan could protect human embryonic hepatocytes against the injury induced by ethanol.

关 键 词：壳寡糖 乙醇 人胚胎肝细胞 P53蛋白 

分 类 号：R285.5[医药卫生—中药学] Q78[医药卫生—中医学]