章丘大葱子叶再生体系建立及再生株倍性鉴定  

Constitute of Regeneration System of Spring Onion Via Cotyledon and Judge of Tetraploid Plantlets

作　　者：穆春华[1] 刘霞[1] 于元杰[2] 王金辉 

机构地区：[1]山东省农业科学院玉米研究所,济南250100 [2]山东农业大学农学院,泰安271018 [3]聊城市东昌府区农业局技术站,聊城252000

出　　处：《中国农学通报》2008年第8期355-359,共5页Chinese Agricultural Science Bulletin

摘　　要：以章丘大葱子叶为材料,研究了不同激素配比对愈伤组织诱导和继代、芽分化、芽点的伸长及根诱导的影响,并对获得的再生植株进行了鉴定。结果表明,诱导章丘大葱子叶产生愈伤组织、愈伤组织的继代培养、愈伤组织芽分化、芽点的伸长及诱导生根的最佳培养基及激素配比分别为:MS+2,4-D2.0mg/L+KT0.5mg/L、MS+2,4-D2.5mg/L+KT0.5mg/L、MS+2,4-D0+KT1.0mg/L、MS+KT0.5mg/L和MS基本培养基;试管苗经驯化移栽后,共有22株成活,成活率为92%,对成活的再生植株进行形态学、叶片气孔及根尖染色体鉴定表明,共有3株发生变异,其中2株为外部形态上的变异,而另一株为染色体的加倍变异。Zhangqiu spring onion was used as experimental materials.We studied the optimum medium and hormone combination of callus induction from cotyledons,subculture of callus,shoot regeneration from callus,shoot-extension induction,root induction and identified the chromosome number of the plantlet.The results showed that：MS＋2,4-D 2.0mg/L＋KT 0.5mg/L,MS＋2,4-D 2.5mg/L＋KT 0.5mg/L,MS＋2,4-D 0＋KT 1.0mg/L,MS＋KT 0.5 mg/L and basal MS medium.One plantlet among twenty two was determined as a tetraploidy based on the plant morphology,observation of the leaf stoma and chromosome number of the root,other two plantlets represented morphological variation.

关 键 词：大葱 子叶 再生体系 再生植株 

分 类 号：S633.1[农业科学—蔬菜学]