不同种源肿节风反丁烯二酸和异秦皮啶含量研究  

Studies on furmarid acid and isofraxidin content in Sarcandra glabra of different provenances

作　　者：闵芳[1] 斯金平[2] 黄文华[3] 黄华宏[2] 楼少卿[2] 朱观泉 

机构地区：[1]浙江师范大学,浙江金华321000 [2]浙江林学院天然药物研发中心,浙江临安311300 [3]中国医学科学院中国协和医科大学药用植物研究所,北京100094 [4]浙江国镜药业有限公司,浙江龙泉323000

出　　处：《中国中药杂志》2008年第15期1849-1853,共5页China Journal of Chinese Materia Medica

基　　金：浙江省重大科技专项项目(2007C12022)

摘　　要：目的:揭示肿节风反丁烯二酸和异秦皮啶质量分数的变异规律,为肿节风资源利用与药材质量优化提供依据。方法:分别建立异秦皮啶与反丁烯二酸的HPLC测定方法,并测定全国21个不同种源肿节风药材中异秦皮啶与反丁烯二酸的质量分数。结果:不同种源肿节风反丁烯二酸和异秦皮啶质量分数存在显著差异,海拔从低到高,两者质量分数都逐渐减少;与经度、纬度关系密切,其中异秦皮啶质量分数在肿节风自然分布中心区最高;肿节风反丁烯二酸和异秦皮啶质量分数地理变异规律不同药材部位表现不一,茎比叶密切。结论:本研究建立的肿节风反丁烯二酸和异秦皮啶质量分数测定方法简便、准确、重现性好,可用于肿节风药材中此2种成分的质量控制。研究结果基本代表了我国肿节风药材质量及其差异的现状,与传统观点认为"江西、福建、浙江所产肿节风药材质量较佳"一致,建议上述区域列为今后肿节风药材良种选育与基地建设的重点。Objective： To study the content variation of furmarid acid and isofraxidin in Sarcandra glabra from 21 different provenances and provide the basis for resource utilization and quality optimization of S. glabra. Method： HPLC method was developed to determine the contents of furmarid acid and isofraxidin in 330 samples of S. glabra which were collected respectively from 21 different provenances. Result： There were significant differences in the contents of isofraxidin and furmarid acid in S. glabra from different provenances. The contents of isofraxidin and furmarid acid dropped off from low altitude to high altitude, which were also close with longitude and latitude. The content of isofraxidin in S. glabra at central area of natural distribution was the highest. The different parts of the plant had different results, the influence on the contents of the acitive components in stem were more obvious than the leaf. Conclusion： This simple, accurate and reproducible method could be use to determine the contents of furmarid acid and isofraxidin in S. glabra. The results represented the status of medicines quality and difference of Chinese S. glabra. These agreed with the traditional views that the medicines quality of Sarcandra glabra in Jiangxi, Fujian, Zhejiang was better. These provenances were considered as important areas of medicines breeding and bases building on S. glabra in future.

关 键 词：肿节风 HPLC 反丁烯二酸 异秦皮啶 种源 变异规律 

分 类 号：R284.1[医药卫生—中药学]