转基因新技术──脉冲交变电泳转基因  

A New Technique of Transgenosis by PFGE

作　　者：戎锐[1] 钱信果[1] 李振刚[1] 

机构地区：[1]中国科学技术大学生物系,230027

出　　处：《高技术通讯》1997年第11期7-10,共4页Chinese High Technology Letters

基　　金：安徽省科委"九五"攻关项目

摘　　要：利用脉冲突变电场使外源DNA穿过密布于家蚕卵壳的曲折气孔孔道，进入卵质之中。分子杂交实验表明脉冲突变电泳能使外源DNA由气孔进入家蚕卵内。电泳后，卵的存活率为40％左右。此项技术较之通用的显微注射、基因枪等简捷方便，转基因可大批量地进行，而且成活率高。该技术可用于一切具有硬壳的昆虫卵、鱼卵等，甚至可用于植物细胞、组织（不必去细胞壁）的转基因操作。A new technique of making the exogenous DNA pass though the tortuous tunnels of bombyxmud eggs' air-pores (about 5000- 10000 air-pores each egg) by PFGE(Pulsed Field Gel beentrophoresis) is presented. Southern dot blot indicates that the exogenous DNA (9. 5kb) has beentransferred into the egg plasm successfully. Consequently, this is a simple and rapid technique. Itcan be also put to use for transgenosis of all insect eggs or fish eggs, even the plant cells with cellwall.

关 键 词：家蚕 脉冲电泳 转基因 点杂交 

分 类 号：S881.2[农业科学—特种经济动物饲养]