晚期糖基化终产物及其受体对大鼠阴茎海绵体组织中氧自由基代谢的影响  

Effect of advanced glycation end products and its receptor on oxyradical metabolization in rat cavernosum

作　　者：陈岽[1] 单玉喜[1] 戴玉田[2] 

机构地区：[1]苏州大学附属第二医院泌尿外科,苏州215004 [2]南京大学医学院附属鼓楼医院泌尿外科

出　　处：《中国男科学杂志》2008年第7期9-12,15,共5页Chinese Journal of Andrology

基　　金：江苏省"135"工程重点医学人才基金(RC2001002)资助

摘　　要：目的通过观察大鼠阴茎海绵体组织中晚期糖基化终产物(AGEs)及其受体(RAGE)的变化对氧自由基代谢的影响,探讨AGEs在糖尿病性勃起功能障碍(DMED)发生发展中的作用。方法成年雄性SD大鼠60只,随机取40只用于制作糖尿病模型,造模成功的大鼠分为两组:糖尿病(DM)组和糖尿病+氨基胍给药(DM+AG)组;另20只大鼠亦分为两组:正常对照(CO)组和正常对照+氨基胍给药(CO+AG)组;氨基胍(AG)给药组大鼠造模后即在其饮水中按1g/L剂量加入AG。饲养8周后取各组大鼠阴茎海绵体组织,一小段用于免疫组化观察分析AGEs及其受体的分布和表达,剩余部分匀浆后检测AGE-肽(AGE-P)含量、丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性。结果DM组阴茎海绵体组织中AGEs和RAGE的表达、AGE-P含量及MDA含量明显高于CO组(P<0.05),而SOD活性则明显低于后者(P<0.05),而AG则明显减少了DM大鼠阴茎海绵体组织中AGEs和RAGE表达、AGE-P及MDA的生成,增强了SOD活性;CO组与CO+AG组间比较在各项指标上则无明显差异(P>0.05)。结论糖尿病状态下AGEs与RAGE的结合效应可以引起大鼠阴茎海绵体组织中氧自由基的产生增多,抗氧化能力的下降,从而促进DMED的发生发展。Objective To illuminate the role of advanced glycation end products （AGEs） in the development of diabetic mellitus erectile dysfunction （DMED）, and investigate the effect of AGEs and RAGE on oxyradical metabolization in rat cavernosum. Methods Forty rats were randomly taken out from 60 male Sprague-Dawley rats for diabetes rnellitus （DM） models. One group of DM rats was given freely access to water（DM group） and another group was given 1g/L aminoguanidine hydrochtoride strating from the occurrence of DM （DM＋AG group）. Two groups of rats that were not DM acted as age-matched group CO and CO＋AG. After eight weeks cavernosum tissue of rate were harvested for analysis of distribution and expression of AGEs and RAGE, content of AGE-peoptide （AGE-P）, malondialdehyde （MDA） as well as the activity of superoxide dismutase （SOD）. Results Compared with that of CO group, the expression of AGEs, RAGE, the content of AGE-P, MDA in rat cavernosum tissue of DM group were significantly higher P〈0.05, but the activity of SOD cavernosum tissue of ACE-P and MDA were downregulated distinctly under the administration of AG, whereas activity of SOD enhanced; There was no difference between CO and CO＋AG groups in all datas （P〉0.05）. Conclusion In the DM condition, the combination of AGEs and RAGE results in the overproduction of oxyradical in rat cavernosum. All these changes may promote the development of DMED.

关 键 词：糖基化终产物 高级 糖尿病 活性氧 阴茎海绵体 大鼠 

分 类 号：R698.1[医药卫生—泌尿科学]