甘蓝枯萎病病原菌的鉴定  

Identification on pathogen of cabbage wilt disease

作　　者：张扬[1] 郑建秋 谢丙炎[3] 李健强[1] 吴学宏[1] 师迎春 马永军 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193 [2]北京市植物保护站,北京100029 [3]中国农业科学院蔬菜花卉研究所,北京100081 [4]北京市延庆县植保站,北京102100

出　　处：《植物病理学报》2008年第4期337-345,共9页Acta Phytopathologica Sinica

基　　金：教育部新世纪优秀人才支持计划(NCET-04-0128);北京市农业科技重大技术研究专项;公益性行业(农业)科研专项经费项目(nyhyzx07-007)

摘　　要：采用温室接种致病性测定、形态学观察和分子鉴定方法对甘蓝枯萎病病原进行了研究。从北京市延庆县9个甘蓝生产基地采集甘蓝枯萎病病样96份,分离获得来自甘蓝病株根、短缩茎、叶片等器官的30个真菌分离物。经过致病性试验证实,分离物GLW 3和GLW 8为甘蓝枯萎病病原菌。经形态学鉴定,GLW 3为尖孢镰刀菌(Fusarium oxysporum),GLW 8为轮枝样镰刀菌(Fusarium verticillio ides)。利用镰刀菌的核糖体DNA(rDNA)内转录间隔区(internal transcribed spacer,ITS)的保守性和变异性,分别采用真菌通用引物和镰刀菌属及轮枝样镰刀菌特异性引物对GLW 3和GLW 8进行PCR扩增,并将测序结果在GenBank中进行同源性比对分析,分子鉴定与形态学鉴定结果一致。轮枝样镰刀菌作为甘蓝枯萎病的病原菌系首次报道。The pathogens of cabbage wilt disease were identified on the basis of pathogenicity testing, as well as morphological characters and molecular identification. Ninety-six diseased cabbage samples were collected from nine villages of cabbage production area in Yanqing county, Beijing. Thirty isolates of possible pathogenic fungi were isolated from roots, stems and leaves of infected cabbage plants. Bases on the pathogenicity experiment, the results showed that GLW3 and GLW8 were the pathogens of cabbage wilt disease. The morphological identification showed that GLW3 remained with Fusarium oxysporum and GLW8 was identified as Fusarium verticillioides. By using of conservation and variability of the internal transcribed spacer （ITS） of Fusarium ribosome DNA, the ITS sequences of the isolates GLW3 and GLW8 were amplyfied by PCR technique with universal primers of fungi and specific primers of Fusarium genus and F. verticillioides, respectively, those sequences were analyzed by comparing with that in GenBank. The molecular identification indicated the same results with morphology identification. Fusarium verticillioides was first reported as the pathogen of cabbage wilt disease.

关 键 词：甘蓝枯萎病 尖孢镰刀菌 轮枝样镰刀菌 形态学观察 分子鉴定 

分 类 号：S436.35[农业科学—农业昆虫与害虫防治]