基于外壳蛋白基因序列对3种葫芦科作物病毒的分子分析  

Molecular analysis of three cucurbit viruses based on coat protein gene sequences

作　　者：古勤生[1] 田延平[1] 彭斌[1] 刘丽锋[1] 邓丛良[2] 粱新苗[2] 孟娟[1] 

机构地区：[1]中国农业科学院郑州果树研究所,郑州450009 [2]北京出入境检验检疫局,北京100026

出　　处：《植物病理学报》2008年第4期357-363,共7页Acta Phytopathologica Sinica

基　　金：国家重大基础研究“973”前期专项(2005CCA01700)

摘　　要：从山西运城的南瓜(Cucurbita moschata)、河南开封和孟津的西葫芦(Cucurbita pepo)上通过ELISA分别检测到南瓜花叶病毒(Squash mosaic virus,SqMV)、西瓜花叶病毒(Watermelon mosaic virus,WMV)和番木瓜环斑病毒西瓜株系(Papa-ya ringspot virus-Watermelon type,PRSV-W),通过接种分离纯化获得其病毒毒原(CH99/211,CH99/113,CH99/69)。本研究克隆了这3个分离物的外壳蛋白基因,应用DNAStar、Clustal X、Phylip等分析软件将这些序列同已报道的相应序列进行分析并构建系统树。结果发现SqMV可以分为两个组,CH99/211分离物与美国的Arizona分离物接近,应属于Z组;WMV分为两个组,CH99/69同国内黑龙江分离物(WMV-HLJ)和云南分离物(WMV-CHN)最接近,成一小簇;PRSV分成两个组,CH99/113属于Ⅱ组,与国内SP、MZ和BN分离物接近。序列分析表明PRSV和WMV在进化上具有地理相关性。SqMV和PRSV-W外壳蛋白基因序列分析属国内首次报道。SqMV, WMV and PRSV were isolated from infected pumpkin （Cucurbita moschata）and squash （Cucurbita pepo） from Shanxi and Henan province of China respectively. Coat protein genes of WMV, SqMV, PRSV-W were cloned, and analyzed with which reported in the GenBank by DNAStar, Clustal X, Phylip etc. The results showed that there were two clusters for SqMV and WMV respectively： CH99/211 closed to SqMV USA isolate （Arizona） whthin Z group; CH99/69 closed to WMV-HLJ and WMV-CHN isolates; CH99/113 belonged to group Ⅱ of PRSV. Sequences analysis suggested that PRSV and WMV diversified based on geographic original evolution. This is the first report about SqMV and PRSV-W at molecular level from China.

关 键 词：番木瓜环斑病毒 西瓜花叶病毒 南瓜花叶病毒 外壳蛋白基因 分子分析 

分 类 号：S432.41[农业科学—植物病理学]