单体素^1H-MRS推断死亡时间的初步研究  

A preliminary study on estimation of time since death by single-voxel proton ^1H-MR spectroscopy

作　　者：杨天潼[1] 李振伟[2] 刘良[1] 郑娜[1] 

机构地区：[1]华中科技大学同济医学院法医学系,湖北武汉430030 [2]华中科技大学同济医学院附属同济医院放射科,湖北武汉430030

出　　处：《中国法医学杂志》2008年第4期220-222,共3页Chinese Journal of Forensic Medicine

摘　　要：目的检测脑内N-乙酰天门冬氨酸、胆碱复合物及磷酸肌酸和肌酸的变化规律,并探讨其与死亡时间的关系。方法选取12只白兔,利用单体素氢质子磁共振波谱测定死后0.5、1、2、4、6、8、12、16、24h脑内N-乙酰天门冬氨酸、胆碱复合物及磷酸肌酸和肌酸峰下面积;分析其在上述时间点的变化规律,建立与死亡时间相关的回归方程。结果在死后24h内N-乙酰天门冬氨酸、磷酸肌酸和肌酸峰下面积随死亡时间的延长而降低(各时间点比较P<0.05),胆碱复合物在死后24h内无明显变化(各时间点比较P>0.05)。回归方程为:Naa:y=0.7425x2-27.282x+299.984(R2=0.962);Cr:y=0.3234x2-12.007x+210.503(R2=0.986)。结论死后24h内兔脑N-乙酰天门冬氨酸、磷酸肌酸和肌酸呈现出一定的变化规律,其与死亡时间相关程度较高,可用于对一定条件下死亡时间的推断。Objective To investigate the time-dependent relationship among postmortem intervals and postmortem levels of nacetylaspartate （Naa）, total choline （Cho） and creatine （Cr） in rabbit＇s brain by single-voxel proton MR spectroscopy （^1H-MRS）. Methods Naa, Cho and Cr were determined in 12 rabbits＇ brains by ^1H-MRS. The metabolite peak areas of Naa, Cho and Cr were calculated at 0.5, 1,2, 4, 6, 8, 12, 16 and 24h after death with regression equations established . Results Decrease in the metabolite peak areas of Naa and total Cr was noted with the extension of post-mortem interval within 24 hours after death. No variation was found in total Cr. The regression equations were deduced as： y = 0.7425x^2-27. 282x ＋ 299.984 （ R^2 = 0.962 ） for Naa, and y = 0. 3234x2-12. 007x ＋ 210. 503 （ R^2 = 0.986） for total Cr. Conclusion Nacetylaspartate and total creatine level varies time-dependently in the rabbit brain with the extention of postmortem interval, which may be used as parameters for estimating time since death.

关 键 词：法医病理学 死亡时间 磁共振波谱 

分 类 号：D919[医药卫生—法医学]