双黄连的提取精制工艺研究  

Improved Techniques for Extracting Shuanghuanglian

作　　者：张文清[1] 夏玮[1] 那晶[1] 刘书琦[1] 

机构地区：[1]华东理工大学化学系,上海200237

出　　处：《世界科学技术-中医药现代化》2008年第3期66-69,65,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科技部国家"十五"攻关计划项目(99-929-02-03):双黄连片现代化国际化示范研究

摘　　要：采用亚沸态水提,絮凝和醇沉相结合的方法实现双黄连的提取精制,达到提高中药有效成分的目的。得到最佳方案为:经超临界萃取后的100g药材,加入10倍体积的水,在90℃下煎煮2h,过滤,滤液浓缩至80mL,在50℃下,加入1.5%的絮凝剂133mL,搅拌5min,离心,上清液浓缩至16mL,加入95%乙醇至含醇量为80%,过滤,滤液蒸干,得率约为5～6%。This is an improved approach to cut down the needed dosage, extraction, 1.5% chitosan flocculation, and alcohol precipitation using a combined process of sub-boiling water to extract and purify medicinal materials in Shuanghuanglian. It goes through the following procedure： extract medicinal materials from Shuanghuanglian using supercritical-CO2 fluid, before boiling them in water 10 times of the extracts at a temperature of 90℃ for 2 hours. The filtrate is concentrated to 80ml, and combined with 133ml of 1.5% Chitosan flocculating agent at a temperature of 50℃. Stir and centrifuge the compound for 5 minutes, and obtain a concentrated supernate of 16mL. Mix the compound with 95%-alcohol, making the content of ethanol up to 80%. A further filtration and drying process allows an extraction rate up to 5-6 %.

关 键 词：双黄连 壳聚糖 絮凝 醇沉 

分 类 号：R284.2[医药卫生—中药学] R259.631[医药卫生—中医学]