双酚A对青春期雄性大鼠生殖发育的影响  

The Effects of Bisphenol A on Reproduction and Development of Pubertal Male Rats

作　　者：宋清坤[1] 郝卫东[1] 尚兰琴[1] 魏雪涛[1] 蒋建军[1] 邢丽娜[1] 王辉[1] 吴双[1] 赵燕[1] 乔杨峥[1] 郝长付[1] 

机构地区：[1]北京大学公共卫生学院毒理学系,北京100083

出　　处：《癌变．畸变．突变》2008年第4期267-271,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金："十一五"国家科技支撑计划基金(2006BKA02A02)

摘　　要：背量与目的：探讨双酚A对青春期雄性大鼠生殖和发育的影响。材料与方法：在雄性sD大鼠青春期（出生后23—53d）灌胃给予不同剂量（5、50、500mg／kg）的双酚A，观察大鼠体重变化，出生后37d开始检查阴茎包皮完全分离的日期，染毒结束后处死大鼠，取大鼠睾丸、附睾称重同时对其进行病理组织学检查，并检测血清中睾酮的浓度以及睾丸中类固醇激素代谢关键酶基因的表达。结果：500mg／kg双酚A处理组的动物体重明显降低（P〈0．05）；5mg／kg双酚A处理组大鼠的睾丸重量明显增加（P〈0．05）；未观察到睾丸和附睾的明显病理性变化；大鼠阴茎包皮完全分离时间也未见明显改变；各剂量组大鼠血清睾酮浓度与对照组差异无统计学意义（P〉0．05），但剂量反应关系曲线呈倒u型；不同剂量的双酚A可以干扰大鼠睾丸类固醇代谢关键酶的基因表达水平。结论：双酚A可以干扰雄性青春期SD大鼠的类固醇代谢酶的表达。BACKGROUND AND AIM：： To explore the effects of peripubertal exposures to bisphenol A on reproduction and development in male rats. MATERIALS AND METHODS： Sprague-Dawley weaning rats received by gavage 0, 5, 50 or 500 mg/kg/day of bisphenol A from post-natal day （PND） 23 to 53. The onset of puberty （progression of preputial separation） was measured on PND 37. Testis and epididymis were weighed and prepared for histological evaluation. Serum testosterone, and gene expression of steroidogenic enzymes in the testis were measured. RESULTS： A decrease in body weight was found in the 500 mg/kg group rats, but there was an increase of testis weight in 5 mg/kg group. There was no difference of serum testosterone between tested groups and control, but the concentration was higher in 50 mg/kg group than in 500 mg/kg group. The gene expressions of steroidogenic enzymes （P450Scc, 17β-HSD and P450Arom） were altered in all dosage groups. CONCLUSION： Peripubertal exposure to Bisphenol A could induce changes in gene expression of some steroidogenic enzymes in the testis of rats.

关 键 词：双酚A 青春期雄性大鼠 生殖和发育 

分 类 号：R965[医药卫生—药理学]