RASSF1基因在胃癌中的表达及其临床意义  

Expression and Significance of RASSF1 Gene in Human Gastric Carcinoma

作　　者：蔡兆根[1] 于东红[1] 王萍[1] 周蕾[1] 冯振中[1] 

机构地区：[1]蚌埠医学院病理学教研室,安徽蚌埠233003

出　　处：《癌变．畸变．突变》2008年第4期279-283,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：安徽省教育厅自然科学重点项目(KJ2007A098)

摘　　要：背景与目的:探讨RAS相关结构家族基因1(RAS association domain family gene 1,RASSF1)在胃腺癌组织细胞中的表达及其临床意义。材料与方法:用逆转录聚合酶链式反应(RT-PCR)方法检测胃癌BGC-823细胞系、50例胃癌组织及相应癌旁正常组织中RASSF1A、RASSF1B和RASSF1C mRNA的表达,以及30例胃癌高危人群、可疑胃癌病人胃黏膜活检标本中的RASSF1基因表达情况;用PCR法检测上述50例胃癌组织中的幽门螺杆菌(Hp)感染率。结果:RASSF1A在胃癌组织中的转录表达缺失率为52%(26/50),其表达缺失率与胃癌分化程度、淋巴结转移及TNM分期相关(P<0.05),但与其组织学类型无相关性。RASSF1A在胃癌高危人群及可疑胃癌病人胃黏膜活检标本中的表达缺失率为20%(6/30),胃癌组织中Hp感染率为64%(32/50),RASSF1A的表达缺失与Hp感染组之间无相关性(P>0.05)。RASSF1B在胃癌组织中的转录表达缺失率为22%(11/50),RASSF1C则全部表达,RASSF1B和RASSF1C在胃癌高危人群及可疑胃癌病人胃黏膜活检标本中全部表达,并且两者与胃癌的组织学类型、分化程度、TNM分期均无相关性。结论:RASSF1A在胃癌组织中存在较高的表达缺失,RASSF1A的表达与胃癌的临床分期和病理分级具有相关性,RASSF1A在胃癌高危人群及可疑胃癌患者胃黏膜活检标本中存在表达缺失,因此有望作为胃癌早期检测的标志物,RASSF1A的表达缺失与Hp感染之间无相关性,它们可能是两个独立的致病因素。BACKGROUND AND AIM： To evaluate the expression of 3 different transcripts and their clinical significance in human gastric adenocarcinomas. MATERIALS AND METHODS： The mRNA expressions of RASSF1A. RASSF1B and RASSF1C were determined by RT-PCR in gastric carcinomas BGC-823 cell lines, 50 specimens of gastric carcinoma and matched normal tissues and 30 gastric biopsy specimens of high-risk group and those with possible gastric carcinoma. H.pylori in the 50 of gastric carcinomas was tested by PCR technique. RESULTS： Expression of RASSF1A mRNA was abnormally low in BGC-823 cell line, loss of expression rates of RASSF1A and RASSF1B in 50 gastric carcinomas were 52% （26/50） and 22% （11/50）, respectively. RASSF1C mRNA was fully expressed. RASSF1A mRNA expression loss was positively related with histological differentiation degree （P 〈 0.05）, lymph node metastasis （P 〈 0.05） and TNM stages（P 〈 0.05）. There was no relationship between RASSF1A expression and H.pylori infection. The rate of expression loss of RASSF1A was 20% （6/30） in gastric biopsy specimens of high-risk group and patients with possible carcinoma. However, RASSF1B and RASSF1C mRNA were fully expressed in both patient groups. The expression of RASSF1B and RASSF1C mRNA showed no statistical relation with gastric carcinoma clinical pathology indexes. CONCLUSION： RASSF1A gene is related to tumor suppression in gastric carcinoma, and correlated with the clinical pathological stage and the histological differentiation. The expression of RASSF1A was lost in gastroc biopsy specimens of high-risk group and patients with possible carcinoma. So, it could be a possible marker of early gastric carcinoma.There was no relationship between RASSF1A expression and H.pylori infection. Both are thus probably independent etiological factors.

关 键 词：胃肿瘤 RASSF1基因 幽门螺杆菌 逆转录-聚合酶链反应 

分 类 号：R753.2[医药卫生—皮肤病学与性病学]