甲基苯丙胺对体外培养的海马神经细胞的毒性作用  

Toxicity of methamphetamine on hippocampal neurons in vitro

作　　者：陈汉[1] 张戎[1] 丁萍[1] 郑世贤[1] 

机构地区：[1]兰州军区高原边防军事医学教研中心,新疆呼图壁831200

出　　处：《西北国防医学杂志》2008年第4期283-285,共3页Medical Journal of National Defending Forces in Northwest China

摘　　要：目的:通过体外海马细胞培养模型研究MA的作用机制,为MA的研究提供一条新的思路。方法:用浓度分别为0、0.8、1.6、3.2、6.4、12.8mmol/L的MA处理海马神经元48h,用MTT比色法分析细胞存活率,倒置显微镜和电镜观察凋亡的形态学改变,流式细胞仪检测细胞凋亡百分率。结果:MA可剂量依赖性的降低神经元的存活率;倒置显微镜观察可见神经元胞体固缩,突起断裂,网络消失;电镜可见其细胞核明显固缩和凝聚;流式细胞仪检查可见在0.8、1.6、3.2、6.4、12.8mmol/LMA处理海马神经元,细胞的凋亡率与对照组相比均有显著性差异(P<0.01),且随剂量增加其凋亡百分率逐渐增大(P<0.01)。结论:一定浓度的MA对体外培养的海马神经细胞具有细胞毒性,并且诱导神经细胞死亡具有剂量相关效应。Objective. To investigate the mechanism of methamphetamine （MA） - induced neuronal damage with hippocampal neurons in vitro culture. Methods： The hippocampal neurons from rats were treated with MA at 0, 0.8, 1.6, 3.2, 6.4 and 12.8 mmol/L respectively for 48 h. The viability of neurons was examined by MTT assay, and the morphological changes were observed by inverted microscope and electron microscope respectively. Results： MA induced the apoptosis of neurons in dose - dependent manner. There was significant difference between the MA - treated group and the control group in the apoptosis rate of hippocampal neurons （ P 〈 0.01 ）. Conclusion： MA at certain concentration is cytotoxic on cultured hippocampal neurons and can induce apoptosis in dose - dependent manner.

关 键 词：甲基苯丙胺 凋亡 海马神经细胞 

分 类 号：R96[医药卫生—药理学]