尖吻蝮蛇凝血酶的止血作用及其作用机制的研究  

Hemostatic Effect of Hemocoagulase Agkistrodon and Its Mechanism

作　　者：吕慧敏[1] 李长龄[2] 董金婵[1] 张翠兰[1] 周湘艳[1] 

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学研究所,北京100080 [2]北京大学药学院,北京100083

出　　处：《中国实验血液学杂志》2008年第4期883-885,共3页Journal of Experimental Hematology

基　　金：国家863计划项目资助;编号2003AA2Z3157

摘　　要：本研究旨在探讨从国产尖吻蝮蛇蛇毒中分离的尖吻蝮蛇凝血酶(HCA)的止血作用及其作用机制。以兔全血凝固时间和小鼠断尾出血时间为指标评价HCA的促凝与止血作用;以全血凝块溶解和纤维蛋白原裂解为指标探讨HCA的止血作用机制。结果表明,HCA能显著缩短兔全血凝固时间和小鼠断尾出血时间,有明显的剂量依赖关系,其作用与阳性对照药立止血(repitilase)相似。HCA使纤维蛋白原α亚基裂解,其诱导形成的血浆凝块能在尿素溶液中溶解。结论:HCA通过水解纤维蛋白原的α亚基释放出A肽,形成纤维蛋白聚合体而产生止血作用,但不引起凝血因子ⅩⅢ的释放。This study was aimed to investigate the hemostatic effects of hemocoagulase agkistrodon （HCA） and its mechanism. The procoagulative and hemostatic effects of HCA were evaluated by using rabit blood coagulatin time and mouse tail bleeding time ; the mechanisms of HCA hemostatic effect were analyzed by using rabbit blood clot lysis and fibrinogen lysis. The results showed that HCA shortened the rabbit blood coagulation time and the mouse tail bleeding time significantly. The effects are nearly similar to that of positive control （reptilase）, HCA also induced rabbit blood clot lysis and directly hydrolysed the α-chain of fibrinogen, It is concluded that HCA exert its hemostatic effects by hydrolysing the α-chain of fibrinogen, but it is not able to induce production of Ⅷ factor.

关 键 词：尖吻蝮蛇凝血酶 止血作用 纤维蛋白原 

分 类 号：R996.3[医药卫生—毒理学] R619.1[医药卫生—药学]