姜黄素对缺氧HepG2细胞中HIF-1α表达的影响及可能机制  

Effect of curcumin on hypoxia-inducible factor-1 alpha expression in hepatocellular carcinoma cells HepG2 under hypoxia and its possible mechanism

作　　者：侯伟[1] 覃华[1] 刘爽[2] 王颖[1] 刘南植[1] 赵秋[1] 田德安[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院消化内科,湖北省武汉市430030 [2]湖北省疾病预防控制中心卫生监测检验防护所,湖北省武汉市430079

出　　处：《世界华人消化杂志》2008年第21期2354-2358,共5页World Chinese Journal of Digestology

摘　　要：目的:研究姜黄素对缺氧条件下人肝癌细胞HepG2中缺氧诱导因子-1α(HIF-1α)表达的影响,并探讨其可能机制.方法:0、1、2、5、10μmol/L的姜黄素处理缺氧条件下的HepG2细胞6h后,用Western blot免疫印迹法和RT-PCR检测HIF-1α的蛋白及mRNA的表达;0μmol/L,10μmol/L姜黄素,10μmol/L姜黄素+10μmol/LMG-132处理缺氧条件下的HepG2细胞6h后,用Western blot检测HIF-1α和VEGF的蛋白水平.结果:HepG2细胞经1、2、5、10μmol/L的姜黄素处理后,HIF-1α蛋白水平与对照组(0μmol/L姜黄素处理)比较明显降低(F=79.81,P<0.01),且降低程度随着姜黄素处理浓度的增加而变大,但各剂量组HIF-1α的mRNA水平与对照组比较无显著性差异;蛋白酶体抑制剂MG-132可以逆转姜黄素所致的HepG2细胞内HIF-1α和VEGF蛋白表达抑制(F=68.47,83.79,均P<0.01).结论:姜黄素通过蛋白酶体途径,在转录后水平抑制缺氧HepG2细胞的HIF-1α表达.AIM： To study the effect of curcumin on hypoxia-inducible factor-1 alpha （HIF-1α） expression in HepG2 hepatocellular carcinoma cells under hypoxic conditions as well as the possible mechanism. METHODS： HepG2 ceils were treated with different concentrations （0, 1, 2, 5, 10 μmol/L） of curcumin under hypoxic conditions for 6 h. The protein and mRNA levels of HIF-1α were detected by Western blot and reverse transcriptionpolymerase chain reation （RT-PCR） respectively. Then HepG2 cells were treated with 0, 10 μmol/L curcumin, or 10 μmol/L curcumin plus 10 μmol/L MG-132 under hypoxic conditions for 6 h. The protein levels of HIF-1α and vascular endothelial growth factor （VEGF） were detected by Western blot. RESULTS： After HepG2 cells were co-incubated with curcumin （1-10 μmol/L） for 6 h, the protein level of HIF-la decreased significantly as compared with that in the control cells （0 μmol/L curcumin treatment ）, in a dose-dependent manner （F = 79.81, P 〈 0.01）, but the mRNA level of HIF-1α was not affected by curcumin. In the presence of MG-132, a proteasome inhibitor, the HIF-la and VEGF protein levels decreased by curcunmin was restored to the level of control cells （F = 68.47, 83.79, both P 〈 0.01）. CONCLUSION： Curcumin suppresses HIF-la expression at post-transcriptional level in HepG2 cells under hypoxia, probably via proteasomedependent pathway.

关 键 词：姜黄素 肝细胞癌 缺氧 缺氧诱导因子-1 血管内皮生长因子 

分 类 号：R285.5[医药卫生—中药学]