缺氧诱导因子-1α与冠心病患者侧支循环建立的相关性研究  

Relationship between hypoxia-inducible factor-1α and construction of collateral circulation in patients with coronary artery disease.

作　　者：龙家茹[1] 陈宋明[2] 王东明[2] 

机构地区：[1]井冈山大学医学院解剖与组织胚胎学教研室,江西省吉安市343000 [2]汕头大学医学院第一附属医院心内科

出　　处：《中国综合临床》2008年第9期866-868,共3页Clinical Medicine of China

基　　金：广东省自然科学基金资助项目（07008215）

摘　　要：目的 探讨冠心病患者体内单个核细胞缺氧诱导因子-1α（（HIF-1α）表达水平与侧支循环建立程度的相关性。方法 选择冠状动脉造影至少有一支血管狭窄超过70％的冠心病患者96例为冠心病组，其中42例有可见的侧支循环，54例未见侧支循环。另选冠状动脉造影正常患者50例作为对照组。免疫组化和Western blot法检测其外周血单个核细胞HIF-1α蛋白含量。结果 免疫组化和Western blot两种方法检测冠心病组HIF-1α表达水平均明显高于对照组（均P〈0．01），冠心病组中有侧支循环患者比无侧支循环患者HIF-1α表达更多（均P〈0．01），冠状动脉侧支循环程度与HIF-1α表达水平呈正相关（r1=0．78，r2=0．84，均P〈0.01）。结论 冠心病患者外周血单个核细胞HIF-1α表达水平与侧支循环形成明显相关，外周血单个核细胞HIF-1α水平的检测有利于预测冠心病患者侧支循环形成程度。Objective To investigate the relationship between hypoxia-inducible factor-1α（HIF-1α） and construction of collateral circulation in patients with coronary artery disease. Methods Collateral vessels were determined in 96 patients with 970% narrowing of at least one coronary artery without prior revascularization ,42 patients with coronary artery collaterals and 54 patients with no coronary artery collaterals. Another 50 cases with normal coronary arteries were selected as control. The levels of HIF-1α expression in monocytes and lymphocytes were tested by immunohistochemistry （ IHC ） and Western blot. Results Compared to the controls, the patients had higher expression of HIF-1α （ P 〈0.01）. Higher HIF-1α expression was found in patients with collaterals than in those without collaterals （P 〈 0.01）. Positive correlation was observed between the expression of HIF--1α protein and collateral score （r1 =0.78,r2 =0.84,P〈0.01）. Conclusion HIF-1α expression in circulating monocytes and lymphocytes are associated with collateral circulation. Detection HIF-1α might be helpful in the prognosis of patients with coronary artery disease.

关 键 词：冠心病 缺氧诱导因子-1Α 侧支循环 

分 类 号：R541.4[医药卫生—心血管疾病]