罗格列酮对1型糖尿病大鼠残存胰岛保护作用形态学研究  

Protective effects of Rosiglitazone natrium on morphologic injuries of remnant islet in rats with type 1 diabetes mellitus rat.

作　　者：许辉[1] 蒙碧辉[1] 林媛媛[1] 陈洪流[1] 

机构地区：[1]广西医科大学第一附属医院内分泌科,南宁530021

出　　处：《中国综合临床》2008年第9期873-875,共3页Clinical Medicine of China

基　　金：广西科学基金资助项目（桂科自0640130）

摘　　要：目的 评价罗格列酮对1型糖尿病大鼠残存胰岛的保护作用。方法 68只Sprague-Dawley雄性大鼠中随机选取8只作为正常对照组，余下以STZ诱导成模。正常对照组仅注射等量的枸橼酸钠缓冲液。成模组（48只）随机分为罗格列酮治疗组24只，糖尿病模型组24只，随后罗格列酮组开始以5mg／（kg·d）罗格列酮混悬液灌胃，糖尿病模型组和正常对照组同时以等体积生理盐水灌胃。注射STZ后的72h及罗格列酮干预后1、2、4、7周，称重并测定空腹血糖后，每组分别处死大鼠4只，留取胰腺标本，分别采用光镜和电镜观察。结果 与糖尿病模型组相比，罗格列酮治疗组大鼠糖尿病症状显著减轻，血糖水平逐步下降，胰岛形态逐步恢复，B细胞分泌颗粒逐步增加。结论罗格列酮可以减轻和改善1型糖尿病大鼠胰岛形态学损伤，对糖尿病大鼠残存胰岛有一定的保护作用。Objective To observe the morphologic changes of pancreatic islets in diabetic rats and the protective effects of rosiglitazone natrium（RSG） on the remnant islet. Methods 8 rats were randomly selected from 68 male Sprague-Dawley rats for normal control group （ group A） , the rest were administered with STZ, and those with FBG≥16.7mmol/L were served as diabetic models,then the rats were randomly assigned as RSG-treated group （ group B, n = 24 ）. RSG were applied via intragastric administration at a dose of 5mg/（ kg · d） in group B. 72 hours after the administration of STZ and 1-,2-,4 and 7 week after the administration of RSG,4 rats were randomly selected respectively, pancreas specimens were obtained and preserved. The morphologic changes were observed under light microscopes and transmission electron microscope. Results The diabetic symptoms of the rats in groupe B lessened significantly, and the FBG levels were decreased gradually, the morphologic injuries of remnant islet were recovering. The amount of secretory granule in beta cells increased gradually. Conclusion Rosig｜itazone natrium can attenuate morphologie injuries of the islets and protect the remnant islet in type 1 diabetes mellilus rats.

关 键 词：罗格列酮 胰岛 糖尿病 大鼠 超微结构 

分 类 号：R587.1[医药卫生—内分泌]