内含子对真核基因表达调控的影响  

Effects of Pre-mRNA Introns on Regulation of Eukaryotic Gene Expression

作　　者：王悦冰[1] 郎志宏[1] 黄大昉[1] 

机构地区：[1]中国农业科学院生物技术研究所,北京100081

出　　处：《生物技术通报》2008年第4期1-4,8,共5页Biotechnology Bulletin

基　　金：国家自然科学基金(30500039);中央级公益性科研院所基本科研业务费专项资金(中国农业科学院生物技术研究所)资助项目

摘　　要：大多数真核基因都含有非编码的间隔序列——内含子,根据剪接机制的不同,可将内含子分为3类:真核mRNA内含子、自我剪接内含子和真核tRNA内含子。在多数情况下,真核mRNA内含子的存在可以提高基因的表达水平,因为其剪接过程会影响mRNA新陈代谢的多个阶段,包括转录、RNA编辑、pre-mRNA的加工、mRNA的出核运输、翻译和无义衰变等。真核mRNA内含子在真核生物基因表达调控中起着重要的作用,是转基因研究中提高外源基因表达的重要元件之一。就真核mRNA内含子的特性、剪接机制及其对真核基因表达调控的影响作一概述。Most eukaryotic genes are interrupted by one or more noncoding intervening sequences （introns）, Introns are classified into three types based on splicing mechanism： pre-mRNA introns,tRNA introns and self-splicing introns, In many cases,pre-mRNA intron-containing versions can exhibit dramatically high expression profiles,because intron splicing can influence almost all steps of mRNA metabolism including transcription, capping,RNA editing,pre-mRNA processing,translation and decay of mRNA products, Intron has become an important factor in regulation of eukaryotic gene expression and the strategic optimization of transgene expression, In this paper,the features and splicing mechanism of pre- mRNA introns, and the effects of pre-mRNA introns in regulation of eukaryotic gene expression were reviewed.

关 键 词：内含子 剪接 基因表达调控 

分 类 号：Q75[生物学—分子生物学]