通心络胶囊对动脉粥样硬化闭塞症家兔ET、NO、SOD、MDA水平变化的影响  

Effects of Tongxinluo Capsule on ET,NO,SOD and MDA in atherosclerosis obliterans rabbits

作　　者：林成仁[1] 王敏[1] 庄百溪[1] 马雪瑛[1] 刘建勋[1] 

机构地区：[1]中国中医科学院西苑医院实验研究中心,北京100091

出　　处：《中成药》2008年第8期1114-1118,共5页Chinese Traditional Patent Medicine

基　　金：国家"863"计划资助课题(2002AA2Z346B)

摘　　要：目的:观察动脉粥样硬化闭塞症(ASO)家兔ET、NO、SOD、MDA水平的动态变化,并用通心络胶囊(人参、水蛭、全蝎、土鳖虫等)进行干预,探讨其对ASO的作用机制。方法:将家兔随机分为假手术组,模型组,通心络胶囊0.8、0.4、0.2 g/kg剂量组,通塞脉片0.8 g/kg剂量组及来适可0.002 g/kg剂量组;采用机械损伤、免疫损伤及高脂饲料喂养等方法复制家兔髂股动脉粥样硬化闭塞症模型;造模8周开始灌胃给药,实验前、给药前、给药4周、给药8周、给药12周测定血液中ET、NO、SOD、MDA水平,实验结束时造影,并取术侧髂股动脉进行组织病理学检查。结果:通心络胶囊明显减轻术侧后肢髂股动脉管腔狭窄程度,降低血清MDA含量,升高血清SOD活性和NO含量。结论:通心络胶囊具有改善内皮细胞功能障碍、清除自由基、抑制脂质过氧化的作用,提示其防治ASO的机制可能与上述作用有关。AIM： To explore the dynamic changes of ET, NO, SOD and MDA in atherosclerosis obliterans （ASO） rabbits, as well as the effects of Tongxinluo Capsule（ Radix et Rhizoma Ginseng ,Hirudo, Scorpio, Eupolyphaga seu Steleophaga, etc. ） （TXL） and the candidate mechanisms. METHODS： Rabbits were randomly divided into 7 groups： sham, model, TXL （0. 8, 0.4, 0. 2 g/kg）, Tongsaimai tablet （0. 8 g/kg） and Laishike （0. 002 g/kg）. The animal model of ASO was established with a combined method of mechanical trauma, immunologic injury and high fat fodder feeding. Rabbits were administrated the drugs for 8 weeks after surgery. The levels of ET, NO, SOD and MDA were determined at the time points below： pre-experiment, pre-drug administration, 4 weeks after drug administration, 8 weeks after drug administration, 12 weeks after drug administration. The histopathological changes in iliofemoral artery were examined after opacification. RESULTS： TXL significantly extenuated the decrease in the caliber of the iliofemoral artery of the injured hind limb and decreased MDA content while increased SOD activity and NO content in serum. CONCLUSION： TXL might show effects on improving endothelial cell dysfunction, clearing free radicals and antagonizing lipid peroxidation, which might be the candidate mechanisms for the prevention and treatment of TXL for ASO.

关 键 词：动脉粥样硬化闭塞症 通心络胶囊 髂股动脉 内皮细胞 

分 类 号：R285.6[医药卫生—中药学]