肝宁颗粒中大黄所含5种蒽醌苷元的HPLC测定  

Determination of anthraquinone aglycones of Radix et Rhizome Rhei in Ganning Granule by HPLC

作　　者：岳淑梅[1] 冯玲玲 齐潇潇[1] 万绍晖[1] 

机构地区：[1]河南大学药学院,河南开封475004 [2]开封市人民医院,河南开封475000

出　　处：《中成药》2008年第8期1155-1158,共4页Chinese Traditional Patent Medicine

摘　　要：目的：建立肝宁颗粒（大黄、川芎）中大黄的含量测定方法。方法：用HPLC法测定大黄中的5种蒽醌苷元。选用NucleosilODS柱，以甲醇-0．1％磷酸溶液（85：15）为流动相，检测波长430nm。结果：HPLC法5种葸醌苷元的线性范围为芦荟大黄素0．0416～0．2912txg，大黄酸0．0312～0．2598μg，大黄素0．0458～0．3203μg，大黄酚0．0496～0．3472μg，大黄素甲醚0．0206～0．1448μg；5种蒽醌苷元的平均回收率为均在96．6％～98．4％。结论：方法简便，准确，灵敏度高，准确性强，重现性好，建立的方法可用于肝宁颗粒的质量控制。AIM： To determine Radix et Rhizoma Rhici in Ganning Granula（Radix et Rhizoma Rhei, Rhizoma Chuanxiong）. METHODS： In order to determine five anthraquinone aglycones of Radix et Rhizoma Rhei, the HPLC system consisted of Nucleosil ODS column, methand-0. 1% phosphoric acid（85： 15） mixture as mobile phase, with detection wavelength at 430 nm. RESULTS： HPLC showed that the linearity ranges were as follows：aloe-emodin concentration was at the range of 0. 041 6-0. 291 2μg, the rhein concentration was at the range of0. 037 1-0.259 8 μg, the emodin concentration was at the range of 0. 045 8-0.320 3 μg, the chrysophanol concentra-tion was at the range of 0. 049 6-0. 347 2 μg, the physcion concentration was at the range of 0.020 6-0. 144 5 μg. The average recoveries of five anthraquinone aglycones were between 96.6% -98.4%. CONCLUSION ： The meth-od is simple, accurate, sensible and with good repeatability and can be used for quality control of Ganning Granule.

关 键 词：肝宁颗粒 大黄 HPLC 蒽醌苷元 

分 类 号：R927.2[医药卫生—药学]