欧美杨107次生木质部纤维素合酶基因片段的克隆及序列分析  

Cloning and Sequence Analysis of CesA Gene Fragment from Secondary Xylem of Populus euramericana cv. "74/76"

作　　者：薛永常[1] 刘长斌[1] 聂会忠[1] 

机构地区：[1]大连工业大学生物与食品工程学院辽宁省发酵工程重点实验室,辽宁大连116034

出　　处：《辽宁林业科技》2008年第4期14-16,共3页Liaoning Forestry Science and Technology

基　　金：辽宁省自然科学基金(20062146);辽宁省高校发酵工程重点实验室开放课题(2006009)

摘　　要：以欧美杨107次生木质部为材料提取总RNA,克隆得到了一个纤维素合酶基因片段。序列分析表明:该基因序列为883bp,与Populus tremula×Populus tremuloides木质部特异性纤维素合酶基因CesA1和Populus×canescens纤维素合酶基因的相似性均达到99%,与Populus tremuloides木质部特异性纤维素合酶基因CesA3的相似性达到98%。此片段拟表达的氨基酸序列具有纤维素合酶共有的锌指结构和一个不完全的高突变区,证明此DNA片段是纤维素合酶基因的片段,构建了重组质粒,并命名为pMD20-T-CesA1。A fragment of CesA（ Cellulose Synthase） gene was amplified during extracting total RNA by using the secondary xylem of Populus cv. ＂74/76＂ as testing materials. The analysis of sequence showed that： sequence of the amplified DNA fragment was 883 bp, and the similarities of the nucleotide sequence with Populus trenuda × P. trenudoldes CesA 1, Populus canescens cell and Populus trenudoides xylem-specific cellulose synthase （ C, esA3 ） were 99%, 99% and 98% respectively. The amino acid sequence to be expressed by the fragment had a zinc finger structure commonly found from CesA and a incomplete high mutation area, which proved the DNA fragment was parts of CesA gene, and therefore the recombinant plasmid were constructed and being named as pMD20 - T- CesA 1.

关 键 词：欧美杨107 纤维素合酶基因 RT—PCR 

分 类 号：Q78[生物学—分子生物学]