纳米ZnO修饰电极上低电位检测烟酰胺腺嘌呤二核苷酸和乙醇  

Detection of Nicotinamide Adenine Dinucleotide (NADH) and Ethanol at ZnO Nanostructure Modified Electrode with Low Overpotential

作　　者：白红艳[1] 包建春[1] 戴志晖[1] 刘可[1] 

机构地区：[1]江苏省生物功能材料重点实验室南京师范大学化学与环境科学学院,南京210097

出　　处：《化学学报》2008年第15期1786-1790,共5页Acta Chimica Sinica

基　　金：国家自然科学基金(No.20505010);生命分析化学教育部重点实验室开放基金(No.KLACLS07003)资助项目

摘　　要：纳米ZnO修饰玻碳电极在0.23V对烟酰胺腺嘌呤二核苷酸(NADH)的氧化具有很好的催化活性,与裸电极上NADH的氧化电位0.70V相比,该电位降低了0.47V,同时增强了抗干扰能力,并在很大程度上减小了电极污染.以乙醇脱氢酶(ADH)为例,制备了ADH/ZnO修饰电极,可用于脱氢酶底物乙醇的快速、灵敏检测,并具有良好的重现性和稳定性.研究表明纳米ZnO为构建脱氢酶底物的电化学传感器提供了一种新的生物兼容性材料.The good catalytic activity of ZnO nanomaterial to the oxidation of nicotinamide adenine dinucleotide （NADH） was described for biosensing application. Compared to a bare electrode, the modified electrode decreased the oxidation overpotential of NADH for 0.47 V, decreased the interferences and minimized the surface fouling. With alcohol dehydrogenase （ADH） as a model, the proposed biosensor showed rapid and sensitive amperometric responses to ethanol with acceptable preparation reproducibility and stability. Nano-ZnO provided an efficient matrix for development of NADH biosensors and the prepared electrode can be used not only to detect the concentration of NADH in a biochemical reaction, but also used as the potential matrix for the construction of dehydrogenases.

关 键 词：NADH ZnO 电催化氧化 传感器 乙醇脱氢酶 乙醇 

分 类 号：O657.1[理学—分析化学]