^(60)Coγ射线辐照对东方百合不定芽POD同工酶的影响  

Effects of ^(60)Co-γ Ray Radiation on POD Isozymes of Adventitious Bud of Oriental Lily

作　　者：黄海涛[1] 王丹[1] 周丽娟[1] 李卫锋[1] 闻方平[1] 

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621000

出　　处：《北方园艺》2008年第7期162-164,共3页Northern Horticulture

基　　金：中国工程物理研究院军民两用开发技术资助项目(200305)

摘　　要：运用60Coγ射线辐照东方百合离体不定芽,对不定芽不同生长时期组织过氧化物(POD)同工酶酶谱进行分析,结果表明:辐照后POD同工酶酶带数和酶带颜色与对照比较都存在一定差异,两个时期6.08、.0 Gy处理的POD同工酶均出现了一条低分子量酶带,对同工酶进行聚类分析发现高剂量处理(≥4.0 Gy)与对照的相似性较小,差异较大。The adventitious buds of oriental lily were treated with ^60Coγ ray. Then the zymograms of POD isozymes of adventitious buds at different growth stages were analyzed. The results showed that the number and the depth of enzy-rnalic bands of POD in 1he treatments and the control were different. There was a low molecular weight band both in the enzymatic bands of POD o[ adventitious buds which were treated at doses of 6.0 Gy and 8. 0 Gy. Using cluster analysis on the isozymes, found there was a obviously difference between the treatments with high dosage （≥4.0 Gy） and the control.

关 键 词：^60Coγ 辐照 东方百合 POD 同工酶 

分 类 号：S682.2[农业科学—观赏园艺]