草地早熟禾高频再生体系建立  

Establishment of the High-efficient Plant Regenaration System of Poa pratensis L.

作　　者：麻冬梅[1] 杨亚亚[2] 杨赛飞[1] 许兴[1] 

机构地区：[1]宁夏大学农学院,宁夏银川750021 [2]宁夏大学生命科学学院,宁夏银川750021

出　　处：《北方园艺》2008年第7期198-200,共3页Northern Horticulture

基　　金：国家“973”资助项目(2006CB100106)

摘　　要：研究了草地早熟禾愈伤组织的诱导和分化的最适培养基,建立了早熟禾高频率的再生体系。结果表明:品种优异的胚性愈伤诱导率最高,其中含2.0 mg/L 2,4-D和0.2 mg/L6-BA的培养基处理胚性愈伤组织的诱导频率最大,可以达到65.26%。早熟禾品种肯塔基、午夜、优异与新哥莱德最适分化激素浓度与配比分别为6-BA 1.0+KT 0.26、-BA 2.0+2,4-D 0.1、6-BA1.0+2,4-D 0.2、KT 2.0,愈伤组织分化率分别达到27.93%、52.38%、66.67%和60%。早熟禾再生芽在1/2 MS+0.2 NAA培养基上生根率可达到100%。The best culture medium of the callus induction, differentiation were studied. The plant regeneration system has been established. The results showed that embryo callus inducing ratio of Merit was the highest, reached 65.25% on the modified MS medium containing 2.0 mg/L 2,4-D and 0. 2 mg/L 6-BA. The best callus differentiation medium of Ken- tuky,Midningt,Merit and Nuglade was different due to different genotype, The best callus differentiation medium of Kentuky,Midningt,Merit and Nuglade was 6-BA 1.0＋KT 0.2,6-BA 2. 0＋2,4-D 0. 1,6-BA 1.0＋2,4-D 0.2,KT 2.0 in- dividually, the ratio of callus differentiation of the Kentuky,Midningt,Merit and Nuglade was 27.93%,52. 38%,66.67% and 60%. The rate of rooting of Poa pratensis L. on half strength MS medium＋0. 2 NAA reached 100%.

关 键 词：早熟禾 愈伤组织 高频再生体系 

分 类 号：S688.403.6[农业科学—观赏园艺]