共振光散射法测定磺胺类药物  

Determination of sulfonamides using resonance light scattering method

作　　者：梁丽娜[1] 葛广华[1] 董存智[1] 

机构地区：[1]淮北煤炭师范学院化学系,安徽淮北235000

出　　处：《中国卫生检验杂志》2008年第8期1561-1562,共2页Chinese Journal of Health Laboratory Technology

基　　金：安徽省教育厅自然科学基金资助项目(2006KJ127B)

摘　　要：目的：建立测定痕量磺胺类药物的新方法。方法：基于磺胺类药物-亚硝酸根-8-羟基喹啉的重氮化-偶联反应。偶联反应产物使得468 nm处共振散射光强度明显增加,从而建立了测定磺胺类药物的新方法。结果：磺胺、磺胺噻唑、磺胺甲恶唑和磺胺甲嘧啶的线性范围分别是：10～1500μg/L、50～1500μg/L、10～1500μg/L、10～1500μg/L;检出限分别为：8.3、12、7.9、9.1μg/L;回收率为99.9%～101.1%。结论：方法的灵敏度高,线性范围宽,用于复方新诺明中磺胺甲恶唑含量的测定,结果满意。Objective： To develop a new method for the determination of sulfonamides.Methods：Based on the diazo-coupling reaction of sulfonamides-nitrite-8-hydroxyquinoline.The result showed that the coupling production enhanced obviously the intensity of resonance light scattering at 468 nm,a resonance light scattering method was proposed for the determination of sulfonamides.Results：The linearity range for the determination was 10~1500 μg/L for sulfanilamide,50~1500 μg/L for sulfathiazole,10~1500 μg/L for sulfamethoxazole and 10~1500 μg/L for sulfamethazine.The detection limit was 8.3 μg/L for sulfanilamide,12 μg/L for sulfathiazole,7.9 μg/L for sulfamethoxazole and 9.1 μg/L for sulfamethazine.The recovery rate was 99.9%~101.1%.Conclusion：The method is of high sensitivity and wide linear range.It can be applied to the determination of sulfamethoxazole in co-sulfamethoxazole tablet samples with satisfactory result.

关 键 词：磺胺类药物 亚硝酸根 8-羟基喹啉 共振光散射法 

分 类 号：O657.3[理学—分析化学]