哺乳期暴露2,3,7,8-四氯二苯并-p-二噁英的子代小鼠生殖发育以及肺组织CYP1A1水平  

Reproductive Development and Pulmonary CYP1A1 Levels in Mice Offspring Exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) During Lactation

作　　者：谭凤珠[1] 张建军[2] 马聪兴[1] 周晓云[1] 刘世朋[1] 张巍丽[1] 

机构地区：[1]河北医科大学公共卫生学院环境卫生教研室,河北石家庄050017 [2]汕头医学院公共卫生与预防医学教研室,广东汕头515031

出　　处：《环境与健康杂志》2008年第7期587-589,F0003,共4页Journal of Environment and Health

基　　金：广东省自然科学基金资助项目(05301076)

摘　　要：目的探讨哺乳期暴露2,3,7,8-四氯二苯并-p-二噁英(TCDD)对子代小鼠生长发育及细胞色素P4501A1(CYP1A1)表达的影响。方法采用清洁级昆明小鼠,设40μg/kg和20μg/kgTCDD染毒组,并分设相应的两个溶剂对照组及一个动物空白对照组。每组母鼠3只,仔鼠25～28只。母鼠分娩后的第1、3、5天腹腔注射TCDD,子代小鼠通过乳汁暴露于TCDD。观察不同生长期仔鼠的体重改变和生殖系统发育等。仔鼠生后第35天被处死,采用免疫组化法分析肺组织CYP1A1的表达。结果TCDD染毒组的仔鼠体重明显降低,雌性仔鼠的阴道平均开放时间明显缩短,雄性仔鼠的睾丸平均下降时间明显延长。TCDD染毒组的肺泡结构紊乱,炎症细胞浸润,肺泡壁增厚水肿,CYP1A1表达量明显升高,并且雌性仔鼠的肺组织反应比雄性仔鼠严重。而对照组中雌雄间差异没有显著性。结论单一哺乳期暴露于TCDD可使子代小鼠体重下降,雌性仔鼠性成熟提前,雄性仔鼠性成熟延缓。仔鼠肺组织CYP1A1表达明显增加,并存在性别差异。Objective To explore the effect of lactational TCDD exposure alone on growing development and cytochrome P4501A1（CYP1A1） in mice offspring. Methods Mature Kunming mice （clean animal,No.604017） were divided to 2 treatment groups （40 and 20 μg /kg bw TCDD）,2 vehicle controls and 1 normal control. There were 3 maternal mice and 25-28 pups in each group. Maternal mice were administered TCDD by intraperitoneal injection in three times on post-parturition days 1,3,5,and the mice offspring were exposed to TCDD by maternal milk. The changes of body weight and the development of reproductive system were observed. The pups were sacrificed on postnatal days （PND） 35,and CYP1A1 expression in the lungs of mice offspring were measured by immunohistochemistry. Results The average body weight of mice offspring in 2 TCDD treatments decreased significantly from PND14 （P〈0.05）. The average vagina-opening days of female offspring were shortened and the average testes-descending days of male offspring delayed significantly （P〈0.05）. In the lung tissues of 2 TCDD treatments,inflammatory cell infiltrated,alveolar wall thickened,cytoplasm presented deeper yellowish-brown,and the expression of CYP1A1 was increased. The changes in female mice offspring were more obvious than those in male mice offspring. No significant difference was observed between any two groups in vehicle controls and animal control. Conclusion The TCDD exposure alone at lactation can decrease the body weight of mice offspring,promote the sexual development for female offspring,and retard the sexual development for male offspring and can cause the expression of CYP1A1 in the lungs increased with a gender difference.

关 键 词：环境污染物 2 3 7 8-四氯二苯并-p-二噁英(TCDD) 细胞色素P4501A1 肺 哺乳期 小鼠 

分 类 号：R994.6[医药卫生—毒理学]