褪黑素对烧伤脓毒症大鼠肝脏核因子-κB表达的影响  

The influence of melatonin on the expression of NF-κB in liver tissue of burn rat with sepsis

作　　者：高卉[1] 白育庭[1] 周燕红[1] 李军[1] 

机构地区：[1]咸宁学院医学院医学临床技能中心,湖北咸宁437100

出　　处：《实用医药杂志》2008年第8期984-985,共2页Practical Journal of Medicine & Pharmacy

基　　金：湖北省教育厅基金重点资助课题(D200628007)

摘　　要：目的了解褪黑素对烧伤脓毒症大鼠肝脏NF-κB表达的影响,从而探讨其对肝脏的保护作用。方法采用30%Ⅲ度烫伤加内毒素攻击大鼠模拟临床烧伤脓毒症,将36只大鼠随机分为3组(每组12只):正常对照组、烧伤脓毒症组、烧伤脓毒症褪黑素治疗组。用sABC免疫组织化学方法测定肝组织中NF-κB的表达,观察肝脏组织形态学改变。结果烧伤脓毒症组肝组织中NF-κB的表达明显增强,肝细胞形态学损害明显,与正常对照组比较有显著性差异(P<0.01);烧伤脓毒症褪黑素治疗组与烧伤脓毒症组比较肝组织中NF-κB的的表达明显减弱(P<0.01),肝细胞形态学损伤减轻。褪黑素可以降低NF-κB活性,同时明显降低血清ALT、AST含量。结论褪黑素能下调肝组织中NF-κB的表达,减轻烧伤脓毒症时肝脏的急性损伤。Objective Understanding the influence of melatonin on the expression of NF-κB in liver tissue of bum rat with sepsis, so as to explore its protection for liver. Methods 36 rats were randomly into three groups： normal control group, burn sepsis group, burn sepsis with melatonin group, 12 in each. The expression of NF-κB in liver tissue was determined by sABC immunohistochemistry, the morphological changes were observed, and the data were analyzed by computer analysis system respectively. Results The expression of NF-κB in liver tissue in burn sepsis group was significantly higher than that in normal control group （P〈0.01）, the architecture of liver was impaired,（P〈0.01 ）. The expression of NF-κB in liver tissue in bum sepsis with melatonin group was significantly lower than that in bum sepsis group （P〈0.01）, the architectural distortion in liver was alleviated （P〈0.01）. After treatment with melatonin, NF -κB expression were decreased, at the same time serum alanine aminotranferase（ALT） and aspartate tranferase（AST） levels were markedly lowered. Conclusion Melatonin could markedly down-regulate the expression of NF-κB in liver tissue and attenuate acute liver injury in bum rat with sepsis.

关 键 词：脓毒症 NF-ΚB 肝损伤 褪黑素 

分 类 号：R631.2[医药卫生—外科学] R644[医药卫生—临床医学]