乙肝标志物定量检测的临床意义  

The Significance of Fixed Quantity Detection of Hepatitis B Indicators

作　　者：刘云峰 刘中国 杜利容 邓清华[2] 

机构地区：[1]四川省眉山市第三人民医院检验科,四川眉山620020 [2]四川省泸州医学院检验系,四川泸州646000

出　　处：《临床和实验医学杂志》2008年第8期70-71,共2页Journal of Clinical and Experimental Medicine

摘　　要：目的探讨乙肝标志物定量检测的临床意义。方法用时间分辨免疫荧光分析法(TRFIA)对1139名公务员进行两对半定量检测同时用酶联免疫吸附实验(ELISA)对相同的标本进行乙型肝炎病毒表面抗原(HBsAg)定性检测;日常门诊和住院病人3745人用ELISA进行两对半定性检测。结果公务员TRFIA定量检测HBsAg阳性(>0.5ng/ml)率6.4%;ELISA定性检测相同标本HBsAg阳性率6.2%;公务员TRFIA定量检测乙型肝炎病毒表面抗体(HBsAb)阳性(>10mIU/ml)率62.3%;日常门诊和住院病人ELISA定性检测HBsAg、HBsAb阳性率分别为11.8%、44.1%。结论乙肝标志物定量检测的灵敏度、准确性更高,临床意义更广泛,尤其是HBsAg和HBsAb。Objective To discuss the clinical significance of fixed quantity detection of Hepatitis B indicator. Methods A Time - Resolved Fluorolmmuno Assay （TRFIA） method is taken to detect the Hepatitis B of 1 139 officials and at the same time, experimental study of Enzyme - linked Immunosorbent Assay （ELISA） is taken for qualitative HBsAg detection to the same samples. To the daily patients, ELISA method is taken for qualitative Hepatitis B detection. Results The positive rate （ 〉 0.5 ng/ml） of the fixed quantity TRFIA detection of the officials is 6.4% while the positive rate of qualitative HBsAg detection to the same samples is 6.2%. The positive rate （ 〉 10 mIU/ml） of fixed quantity TRFIA detection of HBsAb to the officials is 62.3% while positive rate of the qualitative ELISA detection of HBsAg and HBsAb to the daily patients is 11. 8% and 44.1% respectively. Conclusion The sensitivity and accuracy are higher of the detection to the Hepatitis B indicators and the clinical significance is wider, especially for HBsAg and HBsAb.

关 键 词：乙肝标志物 定量 定性 临床意义 

分 类 号：R446.6[医药卫生—诊断学]