多聚物1诱导自身免疫对大鼠高眼压模型视神经的保护作用  

Protection of autoimmunity induced by copolymer-I on optic nerve： experiment with rat glaucoma models

作　　者：李霞[1] 钱韶红[1] 孙兴怀[1] 

机构地区：[1]复旦大学附属眼耳鼻喉科医院眼科,上海200031

出　　处：《中华医学杂志》2008年第30期2152-2154,共3页National Medical Journal of China

基　　金：上海市科委基础研究重点项目基金资助（04jc14023）

摘　　要：目的探讨多聚物1诱导自身免疫对大鼠高眼压模型视神经的保护作用及其机制。方法高眼压大鼠随机分为2组，分别皮下注射200μg多聚物1和同等剂量的磷酸盐缓冲液（PBS）。荧光金逆行标记视网膜神经节细胞（RGC），在荧光显微镜下进行计数。免疫组织化学方法观察T细胞在视神经及视网膜的聚集情况。结果多聚物1组免疫后17、24、31dRGC存活数目（个／mm^2：2617±17、2588±206、2394±15）均多于PBS组（个／mm^2：2357±37、2277±340、2129±17，P〈0．01、0．05、0．01）。免疫后10、17、24、31d视网膜上聚集的T细胞数多聚物1组（个／mm^2：11．3±2．8、36．7±5．2、33．9±3．0、21．4±5．9）也均多于PBS组（个／mm^2：4．7±3．6、19．7±2．4、15．3±4．0、13．3±4．4，均P〈0．01）。结论多聚物1对大鼠高眼压模型的视网膜神经节细胞RGC有保护作用；高眼压造成的损害引起视网膜处T细胞的聚集，多聚物1能增加聚集于视网膜上的T细胞数量，这可能与多聚物1的视神经保护作用有关。Objective To investigate whether copolymer-1 （Cop-1）, a synthesized analogue of myelin basic protein, can protect the retina ganglion cells （RGCs） and possible mechanism thereof. Methods Vortex veins of rats were ligated to induce an increase of intraocular pressure （IOP） so as to establish glaucoma models. 106 rat models were randomly divided into 2 equal groups： Cop-1 Group undergoing subcutaneous injection of 200μg Cop-1, and phosphate buffered solution （PBS） Group undergoing PBS injection as controls. Fluorogold was used to retrogradely label the RGCs. 10, 17, 24, and 31 days after the immunization some rats were killed with their eyeballs taken out. The fluorogold positive spots were calculated. Anti-rat T cell receptor （TCR） monoclonal antibody was used to mark T cells in the retina. Results 17, 24, and 31 days after the immunization, the RGC density of the Cop-1 Group were （2617 ± 17）/mm^2, （2588 ±206） /mm2, and （2394 ± 15）/mm^2 respectively , all significantly higher than those of the PBS Group [ （2357 ±37）/mm^2, （2277 ±340）/mm^2, and （2129 ± 17）/mm^2 respectively, all P 〈 O. 05 ]. 10, 17,24, and 31 days after the immunization the numbers of T cells in the retina of Cop-1 Groupwere （11.3±2.8）/mm^2, （36.7 ±5.2）/mm^2, （33.9±3.0）/mm^2, and （21.4±5.9）/mm^2, all significantly higher than those of PBS Group （4.7 ±3.6） /mm^2, （19.7 ±2.4）/mm^2, （15.3 ±4.0）/mm^2, and （13.3 ±4.4）/mm^2 respectively, all P〈O. 01）. Conclusion Cop-1 protects RGCs. The injury of RGCs induced by increased IOP results in congregation of T cells. Cop-1 increases the number of T- lymphocyte cells congregating in the retina, which may be related to its neuroprotection.

关 键 词：青光眼 免疫 T淋巴细胞 视网膜 

分 类 号：R686[医药卫生—骨科学]