慢性应激对大鼠冠状动脉粥样硬化病变及相关因素的影响  

A Study on the Impact of Chronic Stress on the Cronary Atherosclerosis and Related Factors in Rats

作　　者：李娜[1] 赵兴蓉[1] 龚雪梅[1] 陈美玲[1] 乐发国[1] 许秀峰[1] 

机构地区：[1]昆明医学院附属第一医院精神科,云南省昆明市650032

出　　处：《中国动脉硬化杂志》2008年第6期449-452,共4页Chinese Journal of Arteriosclerosis

基　　金：云南省教育厅青年教师科研基金(5Y0415C);昆明医学院附属第一医院院内基金(2006YN01)

摘　　要：目的探讨慢性应激对冠状动脉粥样硬化病变及相关因素的影响。方法36只Wister雄性大鼠随机平分为3组,均饲养6周,正常对照组喂食普通饲料;高脂组喂食高脂混合饲料;高脂加应激组除喂食高脂混合饲料外,同时给予限制应激、旋转应激、拥挤应激。高脂组和高脂加应激组在应激开始时一次性腹腔注射维生素D(600,000IU/kg),对照组注射同等剂量生理盐水。在研究6周末测定总胆固醇、甘油三酯、高密度脂蛋白、低密度脂蛋白水平,采用放射免疫测定方法测定血栓素B2、白细胞介素6、肿瘤坏死因子水平,并用HE染色法及油红O染色观察冠状动脉病理变化。结果(1)高脂加应激组的总胆固醇、低密度脂蛋白浓度明显高于对照组(P<0.01);高脂组的总胆固醇、低密度脂蛋白浓度显著高于对照组(P<0.01)。(2)高脂加应激组的血栓素B2、白细胞介素6、肿瘤坏死因子浓度明显高于对照组(P<0.01)及高脂组(P<0.05)。(3)慢性应激与血栓素B2、肿瘤坏死因子、总胆固醇呈正相关(r为0.543、0.354、0.392,P均<0.05)。(4)高脂加应激组和高脂组均形成冠状动脉粥样硬化早期病变,但高脂加应激组病变较高脂组加重。结论慢性应激可促进冠状动脉粥样硬化的发展,并可能通过扰乱脂质代谢、增强细胞因子介导的炎症反应、活化血小板等方面影响冠状动脉粥样硬化的发生和发展。Aim To study the impact of chronic stress on the coronary atherosclerosis and related factors Totally 36 male Wister rats were randomly and averagely divided into three groups, and all groups were fed for 6 weeks ： control group were fed with basic food; the rots in hyperlipidemia group were fed with hyperlipidemia diet; the rots in liyperlipidemia stress group were given hyperlipidemia diet, and were exposed under the different stressors at the same times, ineluding limit, circumvolve and huddle. The mrs in hyperlipidemia group and hyperlipidemia stress group were injected with a single dose of vitamin D （600,000IU/kg） at the beginning of the test , and the eontml group were injeeted saline with the equal capacity. The serum level of TC, TG, HDL and LDL were determined after 6 weeks, the serum level of TXB2, IL-6, and TNF-α were tested synchronously with mdioimmunoassay, and the change of pathology of the coronary artery were observed with HE dyeing method. Results （ 1 ） Level of TC, LDL in hyperlipidemia stress group were obviously higher than those in control group （ P 〈 0.01） ; Compared with control group, level of TC, LDL in hyperlipidemia group were higher （ P 〈 0.01 ）. （2） Level of TXB2, IL-6 and TNF-α in hyperlipidemia stress group were remarkably higher than those in control group （P 〈 0.01）and those in hyperlipidemia group （P 〈 0.05 ）. （ 3 ） TXB2, TNF-α and TC were significantly correlated with ehronie mental stress （ r were 0. 543, 0. 354 and 0. 392, P 〈 0.05）. （4） Forepart pathologieal changes of coronary atheroselerosis were formed in both hyperlipidemia stress group and hyperlipidemia group, but hyperlipidemia stress group group were more obvious. Conclusion Chronie mental stress could promote the development of coronary atheroselerosis, whieh was involved in the formation and development of coronary atheroselerosis by disturbing lipide metabolize, inereasing inflammatory reaction guided by eytokines and elevating platelet aetivation.

关 键 词：内科学 应激 动脉粥样硬化 细胞因子 血栓素 大鼠模型 

分 类 号：R5[医药卫生—内科学]