老年人外周血清可溶性凋亡相关因子及其配体水平的变化与心理应激  

Changes of serum concentration of soluble Fas and Fas ligand in the elderly and their clinical significance

作　　者：胡长春[1] 谢文婷[1] 李婷[2] 叶洪青[3] 陈芝芸[4] 朱婉儿[1] 

机构地区：[1]浙江大学医学院医学心理学教研室,杭州市310058 [2]浙江省嘉兴市康慈医院 [3]浙江省望江山疗养院 [4]浙江省中医院消化病研究室

出　　处：《中华老年医学杂志》2008年第8期591-593,共3页Chinese Journal of Geriatrics

基　　金：浙江省教育厅基金（20040234）;卫生部[竹世]川医学奖学金同学会科研启动基金（I20050041）

摘　　要：目的探讨老年人外周血清可溶性凋亡相关因子（sFas）及其配体（FasL）水平的变化及其意义。方法采用双抗体夹心酶联免疫吸附法（ELISA），检测50例老年人血清sFas和FasL水平．与非老年人进行对照研究．并分析老年人血清sFas、FasL水平与心理应激以及应对方式之间的关系。结果老年组血清sFas水平为（10．6±2．8）ug／L，明显高于非老年组（7．5±2．2）ug／L，差异有统计学意义（P〈0．01）；FasL水平为（34．9±19．8）ug／L，明显低于非老年组（52．9±16．1）ug／L，差异有统计学意义（P〈0．01）。老年组sFas水平与心理应激成正相关（r=0.453，P〈0.01）；与积极应对方式成负相关（r=0．535，P〈0．01）。结论老年人血清sFas、FasL水平异常，提示增龄增加b Fas介导的细胞凋亡，且与个体心理应激状况、应对方式有一定关系，这可能提示慢性应激是老年人免疫衰老的重要原因之一；同时老年人体内还可能存在着Fas／FasL的动态平衡机制。Objective To investigate the changes and the clinical significance of serum concentration of soluble Fas （sFas） and Fas ligand （FasL） in the elderly. Methods Fifty elderly subjects and forty seven adults were recruited. Serum concentration of sFas and FasL were detected by enzyme-linked immunosorbent assay （ELISA）. Psycbosocial stress and coping styles were also evaluated. Results The sFas level of the elderly was significantly higher than that in control group, whereas the FasL level was decreased with ageing （P〈0.01）. A positive correlation between sFas level and psychosocial stress, and a negative correlation between sFas level and positive coping were also found （all P〈0.05）. Conclusions Age-related changes occur in serum sFas and FasL levels. The interaction of sFas/FasL system with psychosocial stress and coping styles seems to play important roles in immunosenescence. Our results also suggest that during aging a subtle balance between sFas and FasL could exist.

关 键 词：应激 心理学 凋亡调节蛋白质类 临床分析 

分 类 号：R686[医药卫生—骨科学]