生脉胶囊对慢性心力衰竭大鼠多种细胞因子的影响  

Influence of Shengmai Capsules on multiple cytokines in rats with chronic heart failure

作　　者：邓元江[1] 梁伟雄[2] 陈云波[2] 程淑意[2] 刘卫英[3] 

机构地区：[1]中山大学附属第一医院针灸科,广州510080 [2]广州中医药大学DME中心 [3]广东药学院附属第一医院中医科

出　　处：《北京中医药大学学报》2008年第6期378-381,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：中国博士后科学基金资助项目(No.2005038175);教育部高等学校博士学科点基金资助项目(No.20050572008);广东省自然科学基金资助项目(No.06300042)

摘　　要：目的探讨生脉胶囊对慢性心力衰竭大鼠细胞因子IL-6、TNF-α、IL-10、TGF-β1的影响。方法将大鼠随机分为假手术组、心衰模型组、生脉胶囊组、卡托普利组、生脉胶囊加卡托普利组,采用腹主动脉缩窄法造成大鼠慢性心力衰竭模型,治疗7周时采用ELISA法测定大鼠心肌IL-6、TNF-α、IL-10和血清TGF-β1的含量。结果①心衰模型组心肌IL-6、TNF-α、TGF-β1的含量均高于假手术组(P<0.05或P<0.01),生脉胶囊组、卡托普利组、生脉胶囊加卡托普利组含量均分别低于心衰模型组(P<0.01或P<0.05),但生脉胶囊加卡托普利组TGF-β1含量低于生脉胶囊组(P<0.05)。②心衰模型组心肌IL-10的含量明显低于假手术组(P<0.01),生脉胶囊组高于心衰模型组(P<0.05)。结论生脉胶囊可降低心肌炎性细胞因子IL-6、TNF-α的含量,增加抗炎细胞因子IL-10的含量,降低促纤维性细胞因子TGF-β1的含量,与抑制胶原的合成代谢、改善慢性心衰心室重构有关。Objective To investigate the influence of Shengmai Capsules on some cytokines,including interleukin-6（IL-6）,tumor necrosis factor-α（TNF-α）,IL-10 and transforming growth factor-β1（TGF-β1）,in rats with chronic heart failure.Methods All rats were randomly divided into the sham-operation group（group 1）,chronic heart failure model group（group 2）,Shengmai Capsules group（group 3）,captopril group（group 4） and Shengmai Capsules combining with captopril group（group 5）.The rat model of chronic heart failure was established by using abdominal aortic coarctation.The contents of IL-6,TNF-α,IL-10 in cardiac muscle and TGF-β1 in serum were detected by using enzyme-linked immunosorbent assay（ELISA） on the 7^th week.Results ① The contents of IL-6,TNF-α and TGF-β1 in group 2 were higher than those in group 1（P〈0.05 or P〈0.01）,those in group 3,4 and 5 were lower than those in group 2（P〈0.01 or P〈0.05）,but the content of TGF-β1 in group 5 was lower than that in group 3（P〈0.05）.② The content of IL-10 in group 2 was lower significantly than that in group 1（P〈0.01）,and that in group 3 was higher than that in group 2（P〈0.05）.Conclusion Shengmai Capsules can reduce the contents of inflammatory cytokines of cardiac muscle（IL-6,TNF-α and TGF-β1）,increase the content of anti-inflammatory cytokine（IL-10）,inhibit the anabolism of collagen and relieve the ventricular remodeling of chronic heart failure.

关 键 词：心力衰竭 生脉胶囊 心室重构 细胞因子 白细胞介素-6 白细胞介素-10 肿瘤坏死因子-α 生长转化因子-β1 大鼠 

分 类 号：R285.5[医药卫生—中药学]