三氧化二砷诱导人肝癌HepG_2细胞凋亡及细胞周期阻滞的研究  

Apoptosis and cell cycle-blockade of human hepatocarcinoma HepG_2 cells induced by arsenic trioxide

作　　者：王晓娟[1] 刘小敏[1] 曾春亚[1] 张杨[1] 梁晓秋[1] 

机构地区：[1]湖南衡阳南华大学肿瘤研究所,湖南421001

出　　处：《北京中医药大学学报》2008年第6期404-407,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：湖南省自然科学基金资助项目(No.06JJ2048)

摘　　要：目的研究三氧化二砷(arsenic trioxide,As2O3)对人肝癌HepG2细胞的生长抑制作用,以及诱导细胞凋亡和对周期分布的影响。方法采用MTT法、DNA ladder检测、流式细胞术等方法检测As2O3对人肝癌HepG2细胞的生长抑制,诱导细胞凋亡以及对细胞周期分布的影响。结果MTT法显示:As2O3对HepG2细胞有明显的生长抑制作用,且具有剂量和时间依赖性。12μmol/L As2O3作用HepG2细胞24、48、72 h后,DNA ladder检测,48、72 h均出现凋亡典型的DNA ladder条带;流式细胞仪分析显示:As2O3处理HepG2细胞24、48、72 h后,凋亡率分别为(9.74±1.22)%、(21.89±2.14)%、(42.72±0.83)%,均显著高于对照组细胞的凋亡率(0.98±0.11)%(P(0.01)。同时,流式细胞仪分析显示,As2O3对HepG2细胞有明显的S期和G2期阻滞作用。结论As2O3对人肝癌HepG2细胞S期和G1期阻滞并诱导细胞凋亡可能是其抗肿瘤的机制之一。Objective To study on the inhibitory effect of arsenic trioxide（As2O3） on the growth of human hepatocarcinoma HepG2 cells,its inducing effect on the apoptosis of HepG2 cells,and its influence on cell cycle-blockade.Methods MTT,DNA laddering test and flow cytometry were used to detect the inhibitory effect of As2O3 on the growth of HepG2 cells,its inducing effect on the apoptosis of HepG2 cells,and its influence on cell cycle-blockade.Results In the treatment group MTT outcome showed that As2O3 inhibited significantly the growth of HepG2 cells with a time-dosage dependency.The result of DNA laddering test showed that typical apoptosis bands of DNA ladders were observed after 48 hours and 72 hours when 12 mol·L^-1 As2O3 acting on HepG2 cells for 24,48 and 72 hours.The analysis result of flow cytometry showed that after the action of 12 mol·L^-1 As2O3 for 24,48 or 72 hours the apoptosis rates were（9.74±1.22） %,（21.89±2.14） % or（42.72±0.83） % respectively,which were all higher than that of the control group（0.98±0.11） %（P〈0.01）.At the same time As2O3 had significant blockade effects on the S and G2 phases of cell cycle.Conclusion As2O3 has the blockade effects on the S and G2 phases of cell cycle and can induce cell apoptosis,which may be one of its mechanisms of anti-tumor.

关 键 词：三氧化二砷 肝癌 凋亡 细胞周期 

分 类 号：R285.5[医药卫生—中药学]