新型多孔β-磷酸三钙对羊腔隙性松质骨缺损修复的实验研究  

Repair of Lanucar Cancellous Bone Defects with New Pourous β-Tricalcium Phosphate in Goats

作　　者：李平[1] 王臻[1] 卢建熙 

机构地区：[1]第四军医大学西京医院全军骨科研究所,西安710032 [2]上海贝奥路生物材料有限公司,上海200018

出　　处：《科学技术与工程》2008年第15期4063-4069,共7页Science Technology and Engineering

基　　金：国家自然科学基金(50235020)资助

摘　　要：建立一种新型松质骨缺损的动物模型,观察和评价新型多孔β-磷酸三钙(β-TCP)对该骨缺损的修复能力。成年中国青山羊双侧股骨下端、胫骨上端制备腔隙性松质骨缺损(直径10mm、深20mm),以颗粒型多孔β-TCP(A组)、粉末型多孔β-TCP(B组)进行修复,同时设立冻干异体骨(C组)和空白对照组(D组)。术后4周、12周、24周进行X线片、CT及组织学以观察和评价骨缺损修复情况。结果表明D组不能自行愈合;X线、CT示24周时A、B、C组均骨性愈合,图像数据分析示A组和C组、B组和C组间有统计学差异(P<0.05);组织学示A、B、C三组4周新骨开始长入,24周骨缺损基本修复,4周、12周、24周三组新生骨面积比分别为(6.82±0.84)%、(6.68±1.13)%、(19.98±1.32)%,(16.32±2.21)%、(16.88±2.43)%、(38.63±3.31)%,(37.67±5.52)%、(39.11±6.43)%、(55.67±4.38)%,统计学分析示C组修复效果好于A组、B组(P<0.05),A组、B组间无差异(P>0.05)。该骨缺损模型稳定、可靠,可较好得模拟一些临床中的腔隙性骨缺损环境;新型多孔β-TCP可良好修复该腔隙性松质骨缺损。To design a new model of lacunar cancellous bone defect and assess the osteogenesis of pourous β-tricalcium phosphate（β-TCP） on the model, lacunar cancellous bone defects were established at inferior segments of femurs and superior of tibias bilaterally of each goat. Pourousβ-TCP particle （group A）, pourousβ-TCP powder （group B）, freeze-dried cancellous bone allograft particel（ group C） were filled in bone defects, there was also a group without implant served（group D）. Osteogenesis of each group was evaluated with X-ray, computerized tomo-graphy, histology and histomorphometry at 4th, 12 h and 24th week postoperative. It have found that group D couldn＇t heal spontaneusly throughout 24 weeks. The X-ray density and computer tomography （CT） density measured in all defects of group C were higher than those of groups A and group B （ P 〈 0.05 ）, whereas group A and group B did not differ from each other. Histomorphometrical measurements were performed for the whole defect area at the end of 4th, 12th and 24th week. The bone penetration ratio in group C was higher than group A and group B, groups A and groups B did not differ from each other. Such a stable and reliable model of lacunar cancellous bone defect can supply a similar enviroment which appear in clinical tate new bone in growth, biomaterials degradation and enhance surgeries. The material used in our study can facilibone defect healing in big animal models.

关 键 词：腔隙性骨缺损 多孔β-磷酸三钙 冻干异体骨 

分 类 号：R683[医药卫生—骨科学]