雷帕霉素对Colo-16细胞系Stat3表达的影响  

Effects of Rapamicin on Cell Proliferation and Stat3 Expression in Colo-16 Cell Line

作　　者：魏志平[1] 李圆[1] 刘彦群[1] 

机构地区：[1]徐州医学院附属医院皮肤科,江苏徐州221002

出　　处：《中国皮肤性病学杂志》2008年第8期461-463,共3页The Chinese Journal of Dermatovenereology

摘　　要：目的研究雷帕霉素对皮肤鳞状细胞癌Colo-16细胞系Stat3表达的影响。方法用不同浓度的雷帕霉素处理Colo-16细胞,Hoechst33258荧光染色法观察凋亡细胞的形态学变化;Western-blotting法检测Colo-16细胞Stat3,P-Stat3蛋白表达水平。结果雷帕霉素能够诱导Colo-16细胞凋亡,荧光染色可见细胞核染色质凝聚、核裂解的形态学改变;在雷帕霉素作用后Colo-16细胞Stat3,P-Stat3表达显著下调。结论雷帕霉素能诱导Colo-16细胞凋亡,其作用机制可能与Stat3表达下调有关。Objective To study effect of rapamicin on Stat3 expression in Colo-16 cell line derived from cutaneous squarnous cell carcinoma. Methods Colo-16 cells were treated with different concentrations of rapamycin. The morphological change of apoptotic cells was tested by using the Hoechst 33258 fluorescent staining, The expression of Stat3 and P-Stat3 in Colo-16 cells was detected by Western blotting. Results Raparnycin could induce Colo-16 cells apoptosis, typical morphological changes of apoptosis such an chromatin condensation and nuclei fragments were discovered by fluorescence staining, Stat3 and P-Stat3 expression were significantly reduced after raparnycin intervention. Conclusion Rapamycin can induce Colo-16 cells apoptosis, its mechanism may participate in decreasing the Stat3 expression.

关 键 词：雷帕霉素 皮肤鳞状细胞癌 Colo-16细胞 Star3 P-Star3 

分 类 号：R965[医药卫生—药理学]