鸡β-防御素基因的克隆、序列分析及其在组织中的分布  

Cloning,Sequencing and Distribution of Chick β-defensins Genes

作　　者：马得莹[1] 刘胜旺[2] 李一经[3] 单安山[1] 

机构地区：[1]东北农业大学动物科学技术学院动物营养研究所,哈尔滨150030 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室禽病室,哈尔滨150001 [3]东北农业大学动物医学院,哈尔滨150030

出　　处：《畜牧兽医学报》2008年第8期1033-1039,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30600435)资助;黑龙江省教育厅重点研究项目(1153lZ05);NOVUS国际科研基金

摘　　要：本研究采用RT-PCR法,分别从鸡肝脏和舌组织中扩增到鸡β-防御素(Gallinacin,Gal)Gal-6、Gal-8与Gal-9基因,经序列测定表明,Gal-6、Gal-8和Gal-9大小分别为201、204和201 bp,其成熟肽分别由67、68和67个氨基酸残基组成。组织表达分析表明,Gal-6基因在鸡体内各组织器官中广泛地表达,在鸡皮肤、舌、心脏、小肠、胸肌、肝脏、肾脏、法氏囊、脾脏和胰腺中表达量较高;在肺脏、睾丸和骨髓中也有一定量表达。相比之下,Gal-8在鸡体内的表达较为局限,Gal-8基因仅在舌、小肠、肺脏和肝脏中大量表达。Gal-9基因在鸡体内组织中分布也较广泛,在舌和小肠中大量表达,在气管、肺脏、肝脏、肾脏、骨髓和胸腺有少量表达。Three Gallinacin （Gal） genes： Gal-6, Gal-8 and Gal-9 were cloned from liver and tongue of chicks by RT-PCR, respectively. In addition, we have sequenced these genes. The full cDNA length of Gal-6 and Gal-9 consist of 201 bp encoding 67 amino acids, while Gal-8 consist of 204 bp encoding 68 amino acids. Differential gene expression of Gal-6, Gal-9 and Gal-8 has been demonstrated across a panel of tissues in the chick. It has been shown that Gal-6 mRNA was highly expressed in the skin, tongue, liver, heart, small intestine, breast muscle, kidney, bursa of Fabricius, spleen, and pancreas, and moderately expressed in the lung, testis, and borrow marrow. Gal-8 mRNA was highly expressed in the liver, tongue, small intestine and lung of chick. While, Gal-9 mRNA was highly expressed in the tongue and small intestine, and moderately expressed in the trachea, lung, liver, kidney, borrow marrow and thymus of chick.

关 键 词：抗菌肽 Β-防御素 鸡 基因表达 

分 类 号：S831.2[农业科学—畜牧学]