HPLC法测定土霉素片的含量  

Determination of oxytetracycline tablets by HPLC

作　　者：杨开金[1] 

机构地区：[1]安徽省淮南市食品药品检验所,安徽淮南232007

出　　处：《安徽医药》2008年第9期809-810,共2页Anhui Medical and Pharmaceutical Journal

摘　　要：目的建立高效液相法测定土霉素片含量的方法。方法采用Venusil XBP-C18色谱柱,流动相为0.05 mol·L^-1草酸铵钠溶溶液-二甲基甲酰胺-0.2 mol·L^-1磷酸氢二铵溶液（70.5∶25∶4.5）（用氨试液调pH值至8.3）;柱检测波长（λ）280 nm,流速1.0 ml·min^-1,柱温为35℃。结果土霉素在0.005～0.9 g·L^-1范围内线性关系良好,r=0.9999,平均回收率为100.3%,RSD=1.0%（n=9）。结论本方法简便、准确、重现性好,适用土霉素片的质量控制。Aim To establish an HPLC method for the determination of oxytetracycline tablets.Method A Venusil XBP-C18 column was used with 0.05 mol ·L^-1 ammonium oxalate solution-n,n-Dimethylformamide-0.2 mol·L^-1 diammonium hydrogen solution phosphate（70.5∶ 25∶ 4.5）（pH was adjvsted to 8.3）.The detection wavelength was 280 nm.The flow rate was 1.0 ml·min^-1 and column temperature was 35℃.Results The linear range was 0.005～0.9 g·L^-1（r=0.999 9,n=9） for clotrimazole.The average recovery rate was（100.3±1.0）%.Conclusion The method is simple,accurate and repeatable.It can be used for the quality control of oxytetracycline tablets.

关 键 词：高效液相法 土霉素 含量测定 

分 类 号：R927.2[医药卫生—药学]