女性阴道乳杆菌菌种鉴定及分析  

Identification and Analysis on Lactobacillus of Female Vagina

作　　者：何亮[1] 曾忠铭 陈群[3] 

机构地区：[1]深圳市慢性病防治院病原检验科,广东深圳518020 [2]深圳市南山医院,广东深圳518000 [3]广东医学院微生物学教研室,广东湛江524023

出　　处：《中国医学文摘（皮肤科学）》2008年第4期205-206,221,共3页China Medical Abstracts(Dermatology)

摘　　要：目的探讨乳杆菌属各种乳杆菌在女性阴道中的分布差异。方法采用Nugent评分标准对阴道分泌物进行镜检,接种乳杆菌于MRS培养基分离培养,使用API 50 CHL鉴定条码对乳杆菌进行分类鉴定。结果有39例受检者经Nugent评分及微生态学检查诊断为正常,有2例诊断为细菌性阴道病。共鉴定出9个种41株乳杆菌,其中有2株只在厌氧环境中生长,其余均在厌氧、CO2及需氧环境中生长。鉴定菌种包括:嗜酸乳杆菌16株、类干酪乳杆菌7株、卷曲乳杆菌7株、德氏乳杆菌4株、发酵乳杆菌2株、短乳杆菌2株、植物乳杆菌1株、林氏乳杆菌1株、乳杆菌霍氏亚种1株。结论乳杆菌在不同个体生殖道内所分布的菌种不尽相同,通过鉴定分析能更详尽的掌握阴道内正常菌群的分布情况,可为由阴道微生态改变引起的疾病的诊断及治疗提供一定参考。Objective To investigate the distribution of different species of Lactobacillus in female vagina. Methods Nugent standard was used to diagnose the vaginal discharge, MRS agar was used to culture Lactobacillus and API 50 CHL was used to identify Lactobacillus. Results 39 women were diagnosed as the normal while 2 women were the Bacteria Vaginosis patients; 9 species 41 strains Lactobacillus were detected, and two of them grew in anaerobic environment only. The strains include Lactobacillus acidophilus （16）, Lactobacillus paracasei （7）, Lactobacillus crisparus （ 7 ）, Lactobacillus delbrueckii （4）, Lactobacillus fermentum （2）, Lactobacillus brevis 1 （2）, Lactobacillus plantarum 2 （1）, Lactobacillus lindneri （1）, Lactobacillus H （1）. Conclusion Different Lactobacillus species were detected in different women. Through detect and identify the Lactobacillus, more details of normal flora in vagina were recognized. It provides some feasibility methods to diagnose and therapy the vaglnosis caused by micro ecologic changed.

关 键 词：阴道 正常菌群 乳杆菌 API鉴定条码 分类 

分 类 号：R378.99[医药卫生—病原生物学]