CaSfl1 plays a dual role in transcriptional regulation in Candida albicans  

作　　者：ZHANG TingTing LI Di LI WanJie WANG Yue SANG JianLi 

机构地区：[1]Key Laboratory of Cell Proliferation and Regulation of Ministry of Education, Beijing Normal University, Beijing 100875, China [2]Candida albicans Molecular and Cell Biology Laboratory, Institute of Molecular and Cell Biology, Proteos, 61 Biopolis Drive, Singapore 138673, Singapore

出　　处：《Chinese Science Bulletin》2008年第17期2624-2631,共8页

基　　金：the Joint Research Fund for Overseas Natural Science of China (Grant No. 30228001);National Basic Research Program of China (Grant No. 2007CB914401)

摘　　要：As a newly identified transcription factor in Candida albcians, CaSfl1 has been shown to be involved in cell flocculation and filamentation and in the negative regulation of several genes involved in hyphal growth. In this study, we constructed Casfl1△/△ mutants and confirmed that deletion of this gene indeed affected cell flocculation and filamentation. In addition, by RT-PCR we found that while Casfl1 repressed the expression of several hypha-specific genes including HWP1, ECE1, ALS1, ALS3, and FLO8, it strongly activated the expression of the heat-shock protein genes HSP30 and HSP90 under certain stress conditions. Therefore, we propose that CaSfl1 can act as both positive and negative regulators, thereby playing a dual role in transcriptional controls in Candida albicans.As a newly identified transcription factor in Candida albcians, CaSfll has been shown to be involved in cell flocculation and filamentation and in the negative regulation of several genes involved in hyphal growth. In this study, we constructed Casfl1△/△ mutants and confirmed that deletion of this gene indeed affected cell flocculaUon and filamentation. In addition, by RT-PCR we found that while Casfll repressed the expression of several hypha-specific genes including HWP1, ECE1, ALSl, ALS3, and FL08, it strongly activated the expression of the heat-shock protein genes HSP30 and HSP90 under certain stress conditions. Therefore, we propose that CaSfi1 can act as both positive and negative regulators, thereby playing a dual role in transcriptional controls in Candida albicans.

关 键 词：假丝酵母 CaSFL1 基因注入 形态发生 转录规则 

分 类 号：Q949.326.1[生物学—植物学]