The mobilization of rat’s mesenchymal stem cells into peripheral blood by LiCL and its potency differentiation  

作　　者：DENG dun ZOU ZhongMin ZHOU TaoLi AI GuoPing WANG JunPing DONG ShiWu SU YongPing 

机构地区：[1]Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Department of Preventive Medicine, Third Military Medical University, Chongqing 400038, China [2]Department of Pathogenic Biology, Third Military Medical University, Chongqing 400038, China

出　　处：《Chinese Science Bulletin》2008年第17期2632-2638,共7页

基　　金：the National Key Basic Research and Development Plan of China (Grant No. 2005CB522605);Opening Project of State Key Laboratory of Trauma, Burn and Combined Injury (Grant No. SKLKF200809)

摘　　要：Mesenchymal stem cells (MSCs) are multipotent cells, which can differentiate into different tissues. It is still controversial whether MSCs can be isolated from adult peripheral blood (PB) under normal conditions and whether they can be mobilized in a way similar to that of hematopoietic stem cells (HSCs). In this study, rat MSCs circulating in the PB under normal conditions can be isolated and cultured, and MSCs from PB and BM can be mobilized by LiCL. The mobilized MSCs can be induced to differentiate into neuron by such factors as β-mercaptoethanol and supernatants of nerve cell cultures. The present study provides a broader perspective on the repair of neural trauma.Mesenchymal stem cells （MSCs） are multipotent cells, which can differentiate into different tissues. It is still controversial whether MSCs can be isolated from adult peripheral blood （PB） under normal conditions and whether they can be mobilized in a way similar to that of hematopoieUc stem cells （HSCs）. In this study, rat MSCs circulating in the PB under normal conditions can be isolated and cultured, and MSCs from PB and BM can be mobilized by LiCL. The mobilized MSCs can be induced to differentiate into neuron by such factors as β-mercaptoethanol and supernatants of nerve cell cultures. The present study provides a broader perspective on the repair of neural trauma.

关 键 词：间叶细胞干细胞 外周血 LICL 动物实验 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]