Effects of temperature on the expression of STN7 andphosphorylation of LHCII proteins in Arabidopsis thaliana  

Effects of temperature on the expression of STN7 an phosphorylation of LHCII proteins in Arabidopsis thaliana

作　　者：HU Yuan GAO Xiu JIANG JiaHong RAN Yan DU LinFang 

机构地区：[1]Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, China [2]Institute for Nanobiomedical Technology and Membrane Biology, Sichuan University, Chengdu 610061, China

出　　处：《Chinese Science Bulletin》2008年第17期2646-2651,共6页

基　　金：the National Natural Sciences Foundation of China (Grant No. 30270124);Doctoral Foundation of Ministry of Education of China (Grant No. 20020610094);Program for New Century Excellent Talents in University (Grant No. NCET-04-0861);Sichuan University Research Grant 985

摘　　要：In Arabidopsis thaliana, STN7 kinase is required for phosphorylation of LHCII and for state transitions. In this paper, a hydrophilic polypeptide, derived from the amino acid sequence of STN7, was conjugated to a carrier protein, bovine serum albumin (BSA), to obtain the polyclonal antibody. Immunogenicity and specificity of the polyclonal antibody were evaluated by agar gel immunodiffusion (AGID) test and Western blot analysis. The results show that besides the phosphorylation of LHCII proteins, also the expression of STN7 was regulated by temperature conditions. In addition, the change tendency of LHCII proteins phosphorylation was not only coherent with expression of STN7 with respect to increasing temperature, but also closely related to state transitions. These results would provide useful information for studying regulatory mechanism of LHCII proteins phosphorylation and expression of STN7.In Arabidopsis thaliana, STN7 kinase is required for phosphorylation of LHCII and for state transitions. In this paper, a hydrophilic polypeptide, derived from the amino acid sequence of STN7, was conjugated to a carrier protein, bovine serum albumin （BSA）, to obtain the polyclonal antibody. Immunogenicity and specificity of the polyclonal antibody were evaluated by agar gel immunodiffusion （AGID） test and Western blot analysis. The results show that besides the phosphorylation of LHCII proteins, also the expression of STN7 was regulated by temperature conditions. In addition, the change tendency of LHCII proteins phosphorylation was not only coherent with expression of STN7 with respect to increasing temperature, but also closely related to state transitions. These results would provide useful information for studying regulatory mechanism of LHCII proteins phosphorylation and expression of STN7.

关 键 词：LHCII蛋白质 磷酸化作用 合成多肽 荧光性 

分 类 号：Q51[生物学—生物化学]