两个水稻DH群体茎蘖消长的发育QTL分析  

QTL analysis for development behavior of culm-tillering dynamic using two different double haploid populations

作　　者：姜华[1] 姜亮[2] 孙焕明[1] 曾龙军[3] 薛大伟[4] 梁国华[1] 

机构地区：[1]扬州大学教育部植物功能基因组学重点实验室,江苏扬州225009 [2]中国农业科学院研究生院,北京100081 [3]中国科学院上海生命科学研究院植物生理生态研究所,上海200032 [4]浙江大学农业与生物技术学院,杭州310029

出　　处：《扬州大学学报（农业与生命科学版）》2008年第2期53-60,共8页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家重点基础研究发展计划项目(973-2005CB102807)

摘　　要：利用分别来源于窄叶青8号(ZYQ8)和京系17(JX17)及春江06(CJ06)和台中本地1号(TN1)2个组合的DH群体(分别称为DH-1和DH-2群体),采用非条件及发育QTL分析方法研究水稻茎蘖消长的遗传控制。其中,DH-1群体定位到条件与非条件QTL共44个,分布于第3、12染色体以外的其他10条染色体上;DH-2群体定位到条件和非条件QTL共70个,分布于第7染色体外其他11条染色体上。通过全基因组比较QTL定位,在2个群体间共确定了10对26个处于相似物理位置的QTL位点,其中有6对14个位点均在相同时期内被检测到,很可能包含有控制茎蘖数的主效数量基因,这些QTL位点在显著性、效应值和贡献率均存在较大差异,对其基因本质仍需进一步深入研究。Two different DH populations, one was derived from a cross between japonica rice JX17 and indica rice ZYQ8 and included 127 lines and the other with 120 lines was derived from a cross between japonica rice CJ06 and indica rice TN1 （herein designated as DH-1 and DH-2, respectively） were used for unconditional and developmental quantitative trait locus （QTL） mapping of culm-tiller-number at different growing stages to investigate the genetic basis of the culm-tillering dynamic of rice. The whole genome comparative QTL mapping was also conducted. 44 conditional and unconditional QTL were detected in 10 chromosomes except chromosomes 3 and 12 from DH-1 population, while 70 conditional and unconditional QTL were detected on 11 chromosomes except chromosome 7 from DH-2 population. And through whole genome comparative mapping, there were 10 pairs of 26 QTLs which were located in the similar physical regions were also identified. The results also showed that 6 pairs of 14 QTL loci were detected in the same development stage, and there might be major quantitative trait loci within these QTL. Although we identified resemblant QTLs in the similar physical region in these two DH populations, because of the inconsistent magnitude, further investigation was still needed for the exact position and the essential of the genes.

关 键 词：水稻 茎蘖消长 加倍单倍体群体 数量性状基因 

分 类 号：S511.032[农业科学—作物学] Q348[生物学—遗传学]