一氧化氮对狗胃和十二指肠移行性复合运动的调节作用  

Regulatory Role of Nitric Oxide in Migrating Motor Complex of Stomach and Duodenum in Dogs

作　　者：杨春敏[1] 周吕[1] 张航[1] 郝维[1] 

机构地区：[1]中国医学科学院中国协和医科大学基础医学研究所生理研究室

出　　处：《基础医学与临床》1997年第6期46-51,共6页Basic and Clinical Medicine

基　　金：国家自然科学基金

摘　　要：应用应力传感器记录狗胃十二指肠移行复合运动（ＭＭＣ）。采用静脉注射和十二指肠的肠系膜动脉插管直接注射药物，观察胃和十二指肠ＭＭＣ的变化。结果表明：（１）在十二指肠ＭＭＣⅠ相时静脉和十二指肠动脉插管注射Ｌ－ＮＡＭＥ，可引起胃和十二指肠持续强烈收缩；（２）静脉灌流Ｌ－精氨酸后胃和十二指肠ＭＭＣⅠ相时间明显延长（ｐ＜０．０５），胃和十二指肠ＭＭＣ的Ⅱ相和Ⅲ相动力指数均低于正常胃和十二指肠ＭＭＣ的水平（ｐ＜０．０５）；（３）在灌流Ｌ－精氨酸背景上，静脉或十二指肠动脉插管注射胃动素，其引起的胃和十二指肠收缩平均振幅均低于对照组；（４）十二指肠动脉插管注射ＴＴＸ可以阻断Ｌ－ＮＡＭＥ的收缩作用。结论：肠神经系统（ＥＮＳ）中的非肾上腺能非胆碱能神经递质）—一氧化氮（ＮＯ）在调节狗胃和十二指肠ＭＭＣ中具有重要作用，可能是维持胃肠ＭＭＣⅠ相的重要递质；在胃动素启动ＭＭＣⅢ相中，ＮＯ可能以合成相对减少的方式参与ＭＭＣ的调节。igrating motor complex (MMC) was recorded by strain gauge transducers implanted on the serosa of antrum and duodenum in conscious dogs. Two silastic catheters used to inject drugs were inserted into a vena jugularis externa and arteria pancreaticoduodenalis superior,perfusing proximal duodenum. The study investigated the influences of intrvenous and intraarterial drugs on MMC. The results showed that:(1)LNAME,an inhibitor of nitric oxide synthase,administered intravenously and intraarterially during phase Ⅰ at duodenum, could initiate continuous contractions in stomach and duodenum. (2) After intravenous Larginine,the duration of phase Ⅰ of MMC in stomach and duodenum was longer than that without Larginine (p<0.05),and motility indexes during phase Ⅱ and during phase Ⅲ in stomach and duodenum significanlty decreased as compared to normal MMC (p<0.05). (3) In the background of perfused Larginine,the mean contraction amplitude induced by exogenous motilin given intravenously or intraarterially was lower than that in control group. (4) Tetrodotoxin injected intraarterially could block the contractions induced by intraarterial LNAME. Conclusions:Nitric oxide (NO) containing nonadrenergic noncholinergic neurons play a siginifcant role in regulating MMC. NO may be an important neurotransmitter which maintains phase Ⅰ of MMC. When motilin initiates MMC Ⅲ, NO may be involved in regulating MMC in a way of relatively decreased biosyntheses.

关 键 词：移行性复合运动 一氧化氮 胃动素 十二指肠 胃 

分 类 号：R333.2[医药卫生—人体生理学] R338[医药卫生—基础医学]