脐血CD_(34)^+细胞免疫表型的分析  

Phenotypic analysis of CD_(34)^+ cells from umbilical cord blood

作　　者：董文川[1] 高晖[1] 喻新建[1] 陈珊珊[1] 陆道培[1] 

机构地区：[1]北京医科大学人民医院血液病研究所,100044

出　　处：《北京医学》1997年第6期342-344,共3页Beijing Medical Journal

摘　　要：应用免疫磁珠法分离脐血CD细胞，以比较CD细胞亚群及各系相关抗原的变化。经过分离，CD细胞由1．47±0．77％升至57．39±16．17％，分离后CDCD细胞占CD细胞的比率为7．82±4．79％CD。细胞纯化同时可减少CD细胞达14．12±9．18倍，CD细胞减少16．42±21．47倍；B系（CD(19)）、巨核系（CD(41)）、NK系（CD(56)）各抗原表达率在CD细胞分离后均明显降低。结论：CD细胞纯化可明显减少CD细胞，应用纯化的CD细胞进行移植应加强支持治疗。The CD antigen is expressed by early hematopoietic stem and progenitor cells, itwas detected on the surface of 1.47±0.77% of cord blood mononuclear cells. After positive selection, the final purity of CD cells was 57.39±16.17 %, CDCD/CD ratio was 7.82±4.79%.While CD cells could be depleted 14.12±9.18 folds, the percentage of CD(15),CD(19), CD(41), CD(56)were also reduced. It demonstrated that CD cell separation could be used in T cell depletion, andif CD cells selection was used in clinical transplanation, supportive care was needed.

关 键 词：脐血 CD34^+细胞 流式细胞仪 分离 免疫磁珠法 

分 类 号：R392－33[医药卫生—免疫学]