高效液相色谱法检测乙酰基转移酶表型  

Determination of Acetylation Phenotype by High Performance Liquid Chromatography

作　　者：李倩[1,2,3] 曹建湘 苏敬东 尹松年[1,2,3] 毕文芳 

机构地区：[1]中国预防医学科学院劳动卫生与职业病研究所 [2]北京理化测试中心 [3]中国医学科学院肿瘤研究所

出　　处：《卫生研究》1997年第6期261-263,共3页Journal of Hygiene Research

摘　　要：介绍了一种快速、简便的乙酰基转移酶表型的检测方法。口服１００ｍｇ氨苯砜后３小时采血，用灵敏、特异的高效液相色谱法（分离柱：Ｓｐｈｅｒｉｓｏｒｂ○ＲＯＤＳ－Ｃ１８）分别检测血浆中氨苯砜（ＤＤＳ）及其乙酰化代谢产物单乙酰氨苯砜（ＭＡＤ）的浓度，流动相为水∶乙腈∶冰乙酸＝１０６０∶３００∶２５，紫外检测波长２５４ｎｍ时分离检测效果最好。ＤＤＳ和ＭＡＤ的平均回收率为８４．５％和９０．３％，最小检出浓度分别为０．００１ｍｇ／Ｌ和０．０１ｍｇ／Ｌ。An improved method for rapid and convenient determination of acetylator phenotype was described.The plasma concentrations of dapsone(DDS) and its acetylated metabolic product monoacetyldapsone(MAD) were measured three hours after a single dose oral intake of 100mg dapsone using the specific and sensitive high performance liquid chromatographic assay(Spherisorb ○R ODS C18).It was found that the separating efficiency was high when the mobile phase was composed of water∶acetonitrile∶acetic acid=1060∶300∶25.The detector's wavelength was adjusted to 254 nm and the flow rate was set at 1 0 ml/min.The average recovery ratios of DDS and MAD were 84 5% and 90 3%. The minimum detectable levels are as low as 0 001mg/L for DDS and 0 01mg/L for MAD,respectively.

关 键 词：乙酰基转移酶 表型 高效液相色谱 氨苯砜 

分 类 号：Q555.303[生物学—生物化学]