嗜麦芽寡养单胞菌β-内酰胺酶检测及耐药性分析  

Detection and Assay of Beta-lactamase and Drug Resistance of Stenotrophomonas maltophilia

作　　者：佘军[1] 单才华[1] 

机构地区：[1]杭州市萧山区第一人民医院检验科,浙江杭州311200

出　　处：《中华医院感染学杂志》2008年第8期1158-1160,共3页Chinese Journal of Nosocomiology

摘　　要：目的了解嗜麦芽寡养单胞菌的耐药性,以指导临床用药。方法采用法国生物梅里埃公司VITEK2全自动微生物鉴定仪进行鉴定与药敏试验,双纸片法确证试验与纸片协同法筛选超广谱β-内酰胺酶(ESBLs)和金属β-内酰胺酶(MBL)。结果嗜麦芽寡养单胞菌对亚胺培南天然耐药,对头孢菌素类、氨基糖苷类、氨曲南均高度耐药,对头孢哌酮/舒巴坦、左氧氟沙星、复方新诺明的耐药率最低,分别为12.50%、13.64%、14.77%;产ESBLs菌株占69.32%,产MBL菌株占88.64%,同时产ESBLs与MBL菌株占60.23%。结论嗜麦芽寡养单胞菌对大多数抗菌药物有较高的耐药性,治疗上建议足量联合使用≥2种抗菌药物。OBJECTIVE To study the drug resistance of Stenotrophomonas maltophilia in order to direct clinical drug application. METHODS The S. maltophilia was identified and its sensitivity was analyzed with VITEK2-AMS. The extended-spectrum beta-lactamases（ESBLs） and metallo-lactamase（MBL） were screened by double-disk synergy test and double-disk confirmatory test. RESULTS The resistance rate of S. maltophilia to cefoperazone-sulbactam, levofloxacin and SMZ-TMP was the lowest, accounted for 12. 50%, 13. 64% and 14.77%, respectively. The ESBLs positive rate was 69. 32%, the MBL positive rate was 69. 32%. CONCLUSIONS The multidrug resistance of S. maltophilia is serious and full dose use of two or more antibacterials is recommended.

关 键 词：嗜麦芽寡养单胞菌 超广谱Β-内酰胺酶 金属Β-内酰胺酶 耐药 

分 类 号：R378[医药卫生—病原生物学]