702株假丝酵母菌属感染的菌种鉴定及药敏结果分析  

Identification and Susceptibility Test Analysis of 702 Strains of Candida

作　　者：张妍蓓[1] 何凤莲[1] 徐轲[1] 胡碧丹[1] 徐元宏[2] 

机构地区：[1]安徽医科大学第一附属医院老年呼吸内科,安徽合肥230022 [2]安徽医科大学第一附属医院检验科,安徽合肥230022

出　　处：《中华医院感染学杂志》2008年第8期1195-1197,共3页Chinese Journal of Nosocomiology

基　　金：安徽省自然基金资助(070413068)

摘　　要：目的了解深部真菌感染的病原学分类及药敏试验结果,以探讨预防与治疗措施。方法使用博赛科玛嘉CHROMagar作为假丝酵母菌属分离培养基,DADE WalkAway-40鉴定仪、RY-ID真菌鉴定板鉴定假丝酵母菌属菌种,药敏试验采用ROSCO纸片扩散法。结果共分离出702株真菌,其中白色假丝酵母菌占70.0%,光滑假丝酵母菌占20.8%,热带假丝酵母菌占6.3%;各种真菌对6种抗真菌药物均出现不同程度的耐药。结论真菌感染仍以白色假丝酵母菌为主,但在非白色假丝酵母菌中光滑假丝酵母菌比例有所上升;两性霉素B、氟康唑、伊曲康唑和制霉菌素敏感率高,而5-氟胞嘧啶及酮康唑的耐药率相对高些,临床经验用药应根据药敏结果合理选用。OBJECTIVE To learn the classification of candidal hospital infection and its antifungal drug susceptibility test, in search for prevention and treatment measure. METHODS The CHROMagar candida color medium was applied to isolate Candida, DADE WalkAway-40 and RY-ID were applied to identify Candida strains. Rosco disc diffusion method was applied to analyze susceptibility test of Candida strains. RESULTS Of the 702 isolates, 70.0% were C. albicans, 20.8% were C. glabrata, and 6.3% were C. tropicalis. The candidas showed various resistance to 6 anti-fungal drugs. CONCLUSIONS C. albicans is still the most common pathogen of candidal infection, but in other candida the percentage of C. glabrata is rising now. Amphotericin B, fluconazole, itraconazole and nystatin （Anticandine） have a good antifungal to all candidas, but flucytosine and ketoconazole have less susceptibility to them. So medication should be accorded with the results of antifungal susceptibility test.

关 键 词：假丝酵母菌属 鉴定 敏感性 

分 类 号：R379[医药卫生—病原生物学]